Instrument for determining protein stability

The NanoTemper Prometheus Panta instrument for determining protein stability enables comprehensive characterization of the thermal conformational and colloidal stability of proteins. It combines nano-differential scanning fluorimetry (nanoDSF) to monitor protein unfolding at elevated temperatures by measuring the intrinsic fluorescence of tryptophan residues, with static (SLS) and dynamic light scattering (DLS) to track particle size. SLS allows determination of the aggregation onset temperature, while DLS provides the hydrodynamic radius and particle size distribution. Together, these techniques offer a multidimensional insight into sample stability, which is essential for the characterization of protein samples in research, protein engineering, and quality control.

Responsible for equipment: PhD Miha Pavšič
Equipment is available by prior arrangement with the equipment administrator.