Leica System for non-linear nanoscopy in tandem configuration

An upright nonlinear microscope is used to monitor and quantification of physiological processes in intact tissues and organs. The basis of this microscope enables mounting of biological samples of bigger dimensions and at the same time utilizes deep-penetrating infrared laser light to excite fluorescence. Handling of the laser beam used for fluorescence excitation can be relatively slow to improve the high spatially resolved morphological images or monitoring of relatively slow physiological processes. On the other hand we can move the laser beam over the sample using a high speed mode, which enables monitoring of the physiological processes with millisecond time resolution. The detector side consists of a classical system of high gain photodiodes with a possibility of spectral resolution. The major advance in detection of the faintest signals of fluorescence or bioluminescence comes from the direct mounting the system of non-descanned high resolution photodiodes.

Responsible for equipment: PhD Marjan Slak Rupnik
Prof.Dr. Marjan Slak Rupnik, University of Mariboru, Medical faculty, Ljubljanska 5, 2000 Maribor