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Equipment source: ARIS

Laboratory for sample preparation for transmission electron microscopy

Purpose of equipment
Laboratory for sample preparation for transmission electron microscopy (TEM) includes equipment for preparation of small particles, like viruses, proteins and liposomes with negative staining method as well as preparation of plant and animal tissues and cell cultures for ultrastructure observation. LEICA EM ACE200 is used for preparation of grids for negative staining method and has carbon thread coater for evaporation of carbon of definite thickness and glow discharge unit. Glassknifemaker LEICA EM KMR3 enables preparation of glass knives from glass strips which are used for preparation of semi-thin slices of embedding biological samples using ultracut LEICA ULTRACUT UC7. Light microscope LEICA DM 1000 LED used for observation of semi-thin slices is equipped with digital camera FLEXAM C1 with CMOS sensor. Stereomicroscope LEICA S9 D is also part of the system for preparation of samples for TEM.
Access of equipment
Responsible for equipment: Živa Lengar
URL: http://www.nib.si/infrastruktura/infrastrukturni-center-planta
The equipment is a part of Infrastructural program NIB. For the access of the equipment contact the Head of IC Planta (http://www.nib.si/eng/index.php/infrastructure/infrastructural-centre-planta). Waiting time for the access of the equipment is up to 1 month.
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0105  National Institute of Biology  Ljubljana  5055784  13,297 
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