Projects / Programmes
Introduction of genes resistant to aphids and manifestation of potato necroses caused by viruses
| Code |
Science |
Field |
Subfield |
| 4.03.01 |
Biotechnical sciences |
Plant production |
Agricultural plants |
| Code |
Science |
Field |
| B225 |
Biomedical sciences |
Plant genetics |
| B390 |
Biomedical sciences |
Phytotechny, horticulture, crop protection, phytopathology |
potato, ploidy, Solanum phureja, aphids, interspecific hybrids, PVY, necroses
Researchers (3)
| no. |
Code |
Name and surname |
Research area |
Role |
Period |
No. of publicationsNo. of publications |
| 1. |
13379 |
PhD Peter Dolničar |
Plant production |
Researcher |
1998 - 1999 |
824 |
| 2. |
17304 |
Andrej Ilovar |
|
Researcher |
1998 - 1999 |
6 |
| 3. |
08594 |
Andrej Zemljič |
Plant production |
Researcher |
1999 |
292 |
Organisations (1)
Abstract
The goals of the project are the folowing: induction of dihaploid potato clones from selected Slovene cultivars, study of resistance to aphids and progeny of selected parents, implementation of interspecific crosses between dihaploid potato and wild Solanum species for the introduction of resistance to aphids and study of the incidence of virus necroses on tubers. Slovene potato cultivars were crossed with diploid S. phureja IVP 48 clone. The progeny was sown immediately after the terminated dormancy and ploidy analyzed using flow citometry. From 4 combinations 149 genotypes were raised, 2 of them were diploids and 10 triploids. Their morphological characteristics were described. Genotypes resistant to aphids and fungal and bacterial diseases were obtained from University of Madison in Wisconsin. All genotypes were serologicaly tested to Y, A, X, M, S potato viruses and PLRV by ELISA. Crosses between resistant genotypes and cultivars Sante and Mirakel were made. The incidence of virus necroses on tubers were studied by artificial infection using the method described by Kus et al. The virus isolate was propagated on the cultivar Igor. After the artificial infection the incidence of primary and secondary simptoms of infection on plant and the incidence of necroses on tubers was monitored visually. The plants were tested serologically using ELISA test. The program was conducted at the Agricultural Institute of Slovenia (KIS) at the Field Crop and Seed Production Department and at the Plant Protection Department as well as at the Biotechnical Faculty (BF) at the Agronomy Department, the Centre for Plant Biotechnology and Breeding.
