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Projects / Programmes source: ARIS

Struktura in funkcija skeletne mišice v fizioloških, eksperimentalnih in patoloških pogojih (Slovene)

Research activity

Code Science Field Subfield
3.03.00  Medical sciences  Neurobiology   

Code Science Field
B210  Biomedical sciences  Histology, cytochemistry, histochemistry, tissue culture 
B580  Biomedical sciences  Skeleton, muscle system, rheumatology locomotion 
Keywords
skeletal muscle, myosin heavy chain isoforms, regeneration, immunohistochemistry, electrophoresis, human , rat, mouse, dog, electrical stimulation, motor units, fibre types, hybridisation in situ
Evaluation (rules)
source: COBISS
Researchers (15)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  17930  Ivan Blažinovič    Researcher  1996 - 2001 
2.  10644  PhD Erika Cvetko  Neurobiology  Researcher  2000 - 2001  280 
3.  11785  PhD Vita Čebašek  Neurobiology  Researcher  1999 - 2001  66 
4.  05329  PhD Ida Eržen  Neurobiology  Head  2000 - 2001  212 
5.  07705  PhD Emil Hudomalj  Public health (occupational safety)  Researcher  1996 - 2001  39 
6.  04905  PhD Marija Meznarič  Neurobiology  Researcher  1996 - 2001  152 
7.  17959  Karel Pajk    Researcher  1996 - 2001 
8.  17960  Sonja Petkovšek    Researcher  1996 - 2001 
9.  08010  PhD Samo Ribarič  Neurobiology  Researcher  1996 - 2001  305 
10.  04293  PhD Janez Rozman  Systems and cybernetics  Researcher  2000 - 2001  245 
11.  17961  Marko Slak    Researcher  1996 - 2001 
12.  10641  PhD Viktorija Smerdu  Neurobiology  Researcher  2000 - 2001  91 
13.  09156  PhD Branka Stirn Kranjc  Neurobiology  Researcher  1998 - 2001  386 
14.  17962  Milan Števanec    Researcher  1996 - 2001  21 
15.  17963  Anica Tomažinčič    Researcher  1996 - 2001 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  46,255 
2.  1326  ITIS, Implantable Technology and Sensors, producing, trading, servicing and researching enterprise, d.o.o. Ljubljana  Ljubljana  5824796000  245 
Abstract
In human muscles four groups of fibre types have been defined in regard to MHC-isoform expression, transcripts for the protein and at the enzyme level. Inconsistencies between results obtained at different levels point to muscle fibre type transformations.First studies analysing extensors and flexors of human upper and lower extremity show a good correlation between biomehanical and histochemical characteristics of the same muscles . Expression of myosin heavy chain isoforms has been studied in six month regenerated rat soleus and extensor digitorum longus muscles after muscle excision and transplantation to the extensor digitorum longus muscle bed. Our experimental model compared to other models, described in literature provokes the biggest shift of the MHC isoform profile toward faster MHC-isoforms. Low frequency electrical stimulation of regenerated rat extensor digitorum longus muscle transforms the fast muscle into a muscle with evidently increased proportion of MHC-2a expressing fibres and with up to 20 percents of MHC-1 expressing fibres. In control stimulated EDL muscle the share of MHC-1 expressing fibres did not change. A basis for 3-D reconstruction of the capillary network in rat skeletal muscle has been established and relevant stereological methods for quantification developed. To find the impulse pattern that mimics the physiological one which provokes muscle contraction “in vivo” we determined the fibre type of glycogen depleted fibres in two types of electrical stimulation, with alternating rectangular impulses and with trapezoidal impulses. Both impulse patterns stimulated motor units, consisting of 2A fibres in dog. Levator auris longus muscle of mouse has been used as a model to study sprouting of motor nerve terminals after botulinum A toxin injection. We tested the hypothesis that the two types of sprouting are caused by intrinsic characteristics of slow and fast muscle fibres. The hypothesis has been rejected by immunohistochemical analysis that proved the LAL muscle as a homogeneous fast twitch muscle.
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