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Projects / Programmes source: ARIS

The impact of medicamentous or lifestyle intervention weight loss on endometrial gene expression and protein profile in infertile women with polycystic ovaries syndrome and obesitas

Research activity

Code Science Field Subfield
3.05.00  Medical sciences  Human reproduction   

Code Science Field
B000  Biomedical sciences   

Code Science Field
3.02  Medical and Health Sciences  Clinical medicine 
Keywords
Obesity, polycystic ovaries syndrome, weight loss, endometrial receptivity, biomarkers
Evaluation (rules)
source: COBISS
Researchers (22)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  21364  PhD Helena Ban Frangež  Human reproduction  Researcher  2017 - 2020  194 
2.  28897  Tamara Bertoncelj    Technical associate  2017 - 2020 
3.  33343  PhD Tanja Burnik Papler  Human reproduction  Researcher  2017 - 2020  54 
4.  31347  Sonja Dobrovc    Technical associate  2017 - 2020 
5.  21625  PhD Maja Dolenc  Educational studies  Researcher  2017 - 2020  689 
6.  39347  PhD Marko Hojnik  Oncology  Researcher  2018 - 2019  19 
7.  25612  PhD Nina Jančar  Human reproduction  Researcher  2017 - 2020  131 
8.  22303  PhD Sara Korošec  Human reproduction  Researcher  2017 - 2020  138 
9.  11699  PhD Tea Lanišnik Rižner  Metabolic and hormonal disorders  Researcher  2017 - 2020  574 
10.  23434  PhD Luca Lovrečić  Human reproduction  Researcher  2017 - 2020  168 
11.  17644  Jožica Mivšek    Technical associate  2017 - 2020  65 
12.  22159  Martina Pečlin    Technical associate  2017 - 2020 
13.  17647  Andreja Peterlin    Technical associate  2017 - 2020 
14.  11388  PhD Bojana Pinter  Human reproduction  Researcher  2017 - 2020  791 
15.  34259  PhD Maša Sinreih  Biochemistry and molecular biology  Researcher  2017 - 2020  87 
16.  31842  Margareta Solina    Technical associate  2017 - 2018 
17.  33918  Vesna Šalamun  Human reproduction  Researcher  2017 - 2020  68 
18.  27587  Lijana Šardi    Technical associate  2017 - 2020 
19.  19984  Jožica Šimonka    Technical associate  2017 - 2020 
20.  19985  Majda Štucin    Technical associate  2017 - 2020 
21.  10063  PhD Mateja Videmšek  Sport  Researcher  2017 - 2020  1,244 
22.  12177  PhD Eda Vrtačnik-Bokal  Human reproduction  Head  2017 - 2020  596 
Organisations (3)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0312  University Medical Centre Ljubljana  Ljubljana  5057272000  77,669 
2.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  48,399 
3.  0587  University of Ljubljana, Faculty of Sport  Ljubljana  1627040  19,210 
Abstract
Polycystic ovaries syndrome (PCOS) is the most common endocrinologic disease in reproductive period. It is associated with several reproductive and metabolic disorders. Obesity frequently accompanies PCOS. Obese women with PCOS have lower spontaneous and IVF pregnancy rates, higher miscarriage rates, lower live-born rates and higher complication rates during pregnancy. Causes of infertility in PCOS include anovulation, decreased oocyte quality and reduced endometrial receptivity. It is well known that even a small weight reduction in obese PCOS patients improves endocrine profile, menstrual cycle regularity and fertility thus, weight reduction isconsidered as a standard treatment of PCOS. Embryo implantation represents a crucial step of the reproductive process. Quality embryo, appropriate endometrial receptivity and synchronous dialog between them are needed during the window of implantation (WOI) for successful implantation. Endometrial gene expression of women with high body mass index (BMI) during the WOI differs from gene expression of women with normal BMI, especially if obesity is combined with infertility. Genes related to embryo implantation have been shown to be differentially expressed in these women. It is also known that expression of androgen and estrogen receptor genes in endometrium of overweight women with PCOS differs before and after weight reduction. The purpose of the proposed project is to analyse transcriptome and proteome of endometrial cavity fluid during the WOI and determine whether there are biomarkers of successful embryo implantation in in vitro fertilization (IVF) procedures. Furthermore, we want to determine how the transcriptome and proteome of endometrial cavity fluid of obese (BMI ) 30 kg/m2) women with PCOS during the WOI change after weight loss by using medication or a combination of diet and exercise. We will compare these results with transcriptome and proteome of endometrial cavity fluid during the WOI of women with normal BMI (18.5 – 24.9 kg/m2), who will get pregnant in IVF procedures. With these analyses we want to determine which one of the weight reduction programmes leads towards more phyisiological changes of endometrium on molecular level.
Significance for science
Analysis of gene and protein expression in endometrial fluid before and after weight reduction will help us better understand mechanisms of lower IVF success rates in obese PCOS women. Discovery of biomarkers of endometrial receptivity / WOI would enable us to use them in everyday clinical practice for determination of optimal timing of embryo transfer in each individual patient. The use of biomarkers would lower the number of IVF cycles needed to achieve birth of a child. This would lower financial burden on public healthcare system as six IVF cycles in Slovenia are fully reimbursed by health insurance. It would also lower psychophysical burden of infertile women and couples.   Body weight reduction in obese PCOS women will lower the incidence of obstetric and pregnancy associated complications, maternal and neonatal morbidity and mortality. This will importantly reduce the financial burden of treatment of pregnancy associated complications and lower the need for neonatal intensive care.   The proposed research project represents an original benefit to reproductive medicine as there is a great chance that the results of the study will be used in everyday clinical practice. 17.1. Relevance to the development of science or a scientific field Discovery of biomarkers of endometrial receptivity / WOI would enable us to use them in everyday clinical practice for determination of optimal timing for embryo transfer in IVF procedures for each individual patient. This way IVF procedures would become more individualised. The use of biomarkers would lower the number of IVF cycles needed to achieve a birth of a child. This would lower financial burden on public healthcare system as six IVF cycles in Slovenia are fully reimbursed by health insurance. It would also lower psychophysical burden for infertile women and their partners.
Significance for the country
Weight reduction lowers the incidence of cardiovascular diseases later in life. This lowers the need for the use of antihypertensive medications, medications for treatment of diabetes and dyslipidemia -  conditions that have a higher incidence in obese people. Besides that sick leave costs due to these conditions will be lowered.
Most important scientific results Final report
Most important socioeconomically and culturally relevant results Final report
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