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Projects / Programmes source: ARIS

Pridobivanje keratinolitičnih encimov gliv (Slovene)

Research activity

Code Science Field Subfield
4.06.04  Biotechnical sciences  Biotechnology  Microbe biotechnology 

Code Science Field
T490  Technological sciences  Biotechnology 
P310  Natural sciences and mathematics  Proteins, enzymology 
Keywords
aerobic fermentation, fungi, keratinase, protein purification
Evaluation (rules)
source: COBISS
Researchers (6)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  12667  PhD Miloš Barut  Chemistry  Researcher  1998 - 2001  206 
2.  07850  PhD Marinka Drobnič-Košorok  Veterinarian medicine  Researcher  1999 - 2001  185 
3.  07731  PhD Jožefa Friedrich  Biotechnology  Head  1998 - 2001  184 
4.  17915  PhD Helena Gradišar  Biotechnology  Researcher  1998 - 2001  130 
5.  12728  PhD Aleš Podgornik  Chemical engineering  Researcher  1998 - 2001  709 
6.  17281  Irena Škraba    Researcher  1998 - 2001 
Organisations (3)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0104  National Institute of Chemistry  Ljubljana  5051592000  20,869 
2.  0158  BIA, Laboratory and process equipment company, Ltd.  Ljubljana  5327601  18 
3.  0406  University of Ljubljana, Veterinary Faculty  Ljubljana  1627139  10,704 
Abstract
The production of keratinolytic enzymes will be studied by in vitro cultivation of non-pathogenic fungi in aerobic submerged fermentation. The cultivation conditions will be optimized to obtain active enzymes which will be purified and characterized. The preparative purification will be performed by means of a newly developed method for protein separation on porous compact disks. The purified enzymes will be used in order to obtain specific keratinase antibodies.
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