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Projects / Programmes source: ARIS

Obrambni proteini v rastlinskih celicah (Slovene)

Research activity

Code Science Field Subfield
4.06.05  Biotechnical sciences  Biotechnology  Plant biotechnology 

Code Science Field
B191  Biomedical sciences  Plant biochemistry 
T490  Technological sciences  Biotechnology 
Keywords
cDNA, PVYNTN, insects, virus, proteases, inhibitors, cloning, Agrobacterium, plant, potato, defence, engineering, Colorado potato beetle
Evaluation (rules)
source: COBISS
Researchers (17)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  18343  Aleš Blatnik    Researcher  1998 - 2001  49 
2.  08276  PhD Marjana Camloh  Biotechnology  Researcher  1998 - 2001  155 
3.  12688  PhD Kristina Gruden  Biotechnology  Researcher  1999 - 2001  992 
4.  14804  PhD Marjetka Kidrič  Biochemistry and molecular biology  Researcher  1999 - 2001  118 
5.  04333  PhD Maja Kovač  Biotechnology  Researcher  1998 - 2001  204 
6.  01084  PhD Igor Kregar  Biochemistry and molecular biology  Researcher  1996 - 2001  186 
7.  18344  Lidija Matičič    Researcher  1998 - 2001  42 
8.  19170  PhD Urša Pečar Fonović  Pharmacy  Researcher  1996 - 2001  145 
9.  10263  PhD Nataša Petrovič  Biotechnology  Researcher  1996 - 2001  161 
10.  18467  PhD Maruša Pompe Novak  Biotechnology  Researcher  1996 - 2001  293 
11.  04360  PhD Tatjana Popovič  Biochemistry and molecular biology  Researcher  2000 - 2001  135 
12.  05229  PhD Maja Ravnikar  Biotechnology  Researcher  1998 - 2001  1,374 
13.  05266  PhD Ana Marija Ritonja  Biochemistry and molecular biology  Researcher  1999 - 2001  108 
14.  15813  PhD Boris Rogelj  Neurobiology  Researcher  1996 - 2001  412 
15.  15878  PhD Darja Stanič  Biotechnology  Researcher  1996 - 2001  59 
16.  07849  PhD Borut Štrukelj  Biochemistry and molecular biology  Head  1998 - 2001  1,129 
17.  03765  PhD Jana Žel  Biotechnology  Researcher  1998 - 2001  619 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0106  Jožef Stefan Institute  Ljubljana  5051606000  91,961 
2.  0105  National Institute of Biology  Ljubljana  5055784  13,473 
Abstract
Changes in expression of potato plant defence-related proteins, particulary proteinase inhibitors, as well as certain other pathogenesis-related proteins will be studied at the transcriptional and/or translational level before and after infection with PVYNTN virus, as well as before and after attack. by Colorado potato beetle. As important plant defense-proteins, known and novel proteinase inhibitors will be isolated, characterized and cloned. After testing for their inhibitory activity on PVY virus protease and some insect digestive proteinases, cDNAs corresponding to selected inhibitors will be inserted into potato genom by Agrobacterium-mediated gene transfer and transgenic plants will be generated and tested on the resistance against PVYNTN virus and Colorado potato beetle.
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