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Projects / Programmes source: ARIS

Mikrobna presnova dušika: mineralizacija, nitrifikacija, denitrifikacija (Slovene)

Research activity

Code Science Field Subfield
4.03.02  Biotechnical sciences  Plant production  Soil and micro-climate 

Code Science Field
P310  Natural sciences and mathematics  Proteins, enzymology 
B230  Biomedical sciences  Microbiology, bacteriology, virology, mycology 
Keywords
mineralization, nitrification, denitrification, nitrate reductase, microbial community, soil microbial biomass nitrogen, soil microbial biomass carbon
Evaluation (rules)
source: COBISS
Researchers (6)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  03156  MSc Polonca Čadež  Biochemistry and molecular biology  Researcher  1998 - 2001  64 
2.  17794  Simona Leskovec    Researcher  2000 - 2001  25 
3.  07043  PhD Ivan Mahne  Plant production  Head  1996 - 2001  200 
4.  00529  PhD France Megušar  Plant production  Researcher  1996 - 2001  202 
5.  12466  PhD Alenka Prinčič  Animal production  Researcher  1996 - 2001  26 
6.  13005  PhD David Stopar  Plant production  Researcher  1999 - 2001  469 
Abstract
The research project is focused on microbial metabolism of nitrogen. Periplasmic nitrate reductase in Pseudomonas stutzeri JM300 was partly purified and amino acid sequences of N-terminus was determined to find sequence similarity with a protein of known function in other organisms. Threshold values of oxygen concentration for synthesis and activity of nitrate and nitrite reductases were studied in two selected denitrifying bacteria Pseudomonas stutzeri JM300 and Flavobacterium sp. ATCC 33514. Organic substrate utilization efficiency with O2 or NO3-, as terminal electron acceptors, were measured in experiments with pure cultures of selected denitrifying bacteria. Effects of key environmental factors such as soil pH, oxygen and mineral nutrients on the mineralization capacity of uncultivated peat soil were estimated under laboratory condition. Changes in nitrifying community structure in response to different prevailing ammonium concentrations, pH values and oxygen levels were studied in experimental reactors containing bacterial community from a waste water.
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