Projects / Programmes
Mikrobna presnova dušika: mineralizacija, nitrifikacija, denitrifikacija (Slovene)
Code |
Science |
Field |
Subfield |
4.03.02 |
Biotechnical sciences |
Plant production |
Soil and micro-climate |
Code |
Science |
Field |
P310 |
Natural sciences and mathematics |
Proteins, enzymology |
B230 |
Biomedical sciences |
Microbiology, bacteriology, virology, mycology |
mineralization, nitrification, denitrification, nitrate reductase, microbial community, soil microbial biomass nitrogen, soil microbial biomass carbon
Researchers (6)
no. |
Code |
Name and surname |
Research area |
Role |
Period |
No. of publicationsNo. of publications |
1. |
03156 |
MSc Polonca Čadež |
Biochemistry and molecular biology |
Researcher |
1998 - 2001 |
64 |
2. |
17794 |
Simona Leskovec |
|
Researcher |
2000 - 2001 |
25 |
3. |
07043 |
PhD Ivan Mahne |
Plant production |
Head |
1996 - 2001 |
200 |
4. |
00529 |
PhD France Megušar |
Plant production |
Researcher |
1996 - 2001 |
202 |
5. |
12466 |
PhD Alenka Prinčič |
Animal production |
Researcher |
1996 - 2001 |
26 |
6. |
13005 |
PhD David Stopar |
Plant production |
Researcher |
1999 - 2001 |
469 |
Abstract
The research project is focused on microbial metabolism of nitrogen. Periplasmic nitrate reductase in Pseudomonas stutzeri JM300 was partly purified and amino acid sequences of N-terminus was determined to find sequence similarity with a protein of known function in other organisms. Threshold values of oxygen concentration for synthesis and activity of nitrate and nitrite reductases were studied in two selected denitrifying bacteria Pseudomonas stutzeri JM300 and Flavobacterium sp. ATCC 33514. Organic substrate utilization efficiency with O2 or NO3-, as terminal electron acceptors, were measured in experiments with pure cultures of selected denitrifying bacteria. Effects of key environmental factors such as soil pH, oxygen and mineral nutrients on the mineralization capacity of uncultivated peat soil were estimated under laboratory condition. Changes in nitrifying community structure in response to different prevailing ammonium concentrations, pH values and oxygen levels were studied in experimental reactors containing bacterial community from a waste water.