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Projects / Programmes source: ARIS

Mechanism of glutamate release from astrocytes

Research activity

Code Science Field Subfield
3.03.00  Medical sciences  Neurobiology   

Code Science Field
B640  Biomedical sciences  Neurology, neuropsychology, neurophysiology 
Keywords
exocytosis, glutamate, astrocytes, electrophysiology, capacitance measurements
Evaluation (rules)
source: COBISS
Researchers (4)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  06846  Sonja Grilc  Neurobiology  Researcher  2000 - 2001  57 
2.  18825  PhD Irina Milisav Ribarič  Neurobiology  Researcher  2000 - 2001  154 
3.  12266  PhD Marjan Slak Rupnik  Metabolic and hormonal disorders  Head  1999 - 2001  350 
4.  03702  PhD Robert Zorec  Neurobiology  Researcher  2000 - 2001  802 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  48,429 
Abstract
During the last decade it became clear that glia may play dynamic roles in the nervous system. Rather then being only supporting cells, it is now evident that some glial cells can release neurotransmitters, such as glutamate. However, the mechanism of neurotransmitter release is not known. To explore the hypothesis that calcium-regulated glutamate release is mediated by exocytosis, we will monitor changes in surface membrane area using capacitance measurements. The sensitivity of this technique allows the detection of fusion of unitary synaptic-like vesicles with the plasmamembrane. We will correlate electrophysiological measurements with optically detected glutamate. Clostridial toxins, which target SNARE proteins, will be used to determine whether SNARE proteins are necessary for calcium-dependent glutamate release.
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