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Projects / Programmes source: ARIS

Molekularna biologija v biotehnološih raziskavah nižjih evkariontov (Slovene)

Research activity

Code Science Field Subfield
4.06.01  Biotechnical sciences  Biotechnology  Recombinant DNA technology 

Code Science Field
T000  Technological sciences   
T490  Technological sciences  Biotechnology 
Keywords
Saccharomyces cerevisiae, CDC6, cell cycle, two hybrid system; Aspergillus niger, filamentous fungi, hTNF-alpha, hG-CSF, EK, heterologous gene expression, protein secretion; Cochliobolus lunatus, Rhizopus nigricans, steroid monooxygenase, xenobiotics, plant steroids.
Evaluation (rules)
source: COBISS
Researchers (12)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  16104  PhD Apolonija Bedina Zavec  Biotechnology  Researcher  1999 - 2001  143 
2.  06457  PhD Aleksandra Comino  Biochemistry and molecular biology  Researcher  1999 - 2001  61 
3.  06108  PhD Vladimira Gaberc-Porekar  Biotechnology  Head  1999 - 2001  220 
4.  18672  Mateja Jeras  Biochemistry and molecular biology  Researcher  2000 - 2001  25 
5.  17275  Ana Marija Jesenko    Researcher  1999 - 2001 
6.  06135  PhD Radovan Komel  Biochemistry and molecular biology  Researcher  1999 - 2001  1,053 
7.  00385  PhD Nada Kraševec  Biotechnology  Researcher  1999 - 2001  208 
8.  17276  Jelka Lenarčič    Researcher  1999 - 2001 
9.  16105  MSc Irena Lukančič  Biotechnology  Researcher  1999 - 2001  11 
10.  06707  PhD Aleksander Pavko  Biotechnology  Researcher  1999 - 2001  338 
11.  12200  PhD Marko Vitas  Biochemistry and molecular biology  Researcher  1999 - 2001  77 
12.  11250  PhD Polona Žnidaršič Plazl  Biotechnology  Researcher  1999 - 2001  431 
Organisations (3)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0103  University of Ljubljana, Faculty of Chemistry and Chemical Technology  Ljubljana  1626990  23,092 
2.  0104  National Institute of Chemistry  Ljubljana  5051592000  21,377 
3.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  45,408 
Abstract
The project is composed of three parts: A/ Interactions of Cdc6 protein in the cell cycle of yeast S.cerevisiae: B/ Cloning, expression and secretion of heterologous proteins in the filamentous fungus A.niger; C/ Some aspects of steroid metabolism in filamentous fungi. A/By the “Interaction trap” method, using CDC6 gene product as a bait, four gene products interacting with Cdc6 protein were detected. Most of them are genes with unknown function and none of them has been described to interact with CDC6 yet. Our research will focus on elucidation of interactions of these genes with CDC6, one by one and within the yeast cell cycle. B/ We have already expressed hTNF-alpha gene in A. niger but without secretion into the culture broth. At the same time, preliminary results were obtained on expression and secretion of two other proteins, hG-CSF and bEK. The aim of this research proposal is to apply scientific methods to improve production and secretion of these recombinant proteins for biotechnological application, and to investigate the reasons for impaired processing and secretion of recombinant hTNF-alpha - as a model for research on heterologous protein secretion mechanisms in filamentous fungi. C/ By induction experiments, involvement of fungal steroid monooxygenases in their protecting role against plant steroids and some xenobiotics will be studied. We will also try to clone some monooxygenases by PCR and RACE-PCR approach.
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