Molekularna biologija v biotehnološih raziskavah nižjih evkariontov (Slovene)

Code

J4-1500 (D) - included in ARIS records

Head

PhD Vladimira Gaberc-Porekar

Period

1/1/1999 - 6/30/2001

Science

Natural sciences and mathematics (4)
Biotechnical sciences (6)
Other (2)

Reseacher status

Researcher (10)
Junior expert or technical associate (2)

Education

Doctor's degree (8)
Master's degree (1)
Other (3)

Sex

Woman (9)
Man (3)

Status

Employed at RO and RRD (3)
No data on employment in RO (4)
Retired (4)
Deceased (1)

No. of publications

0 (1)
1–9 (1)
10–99 (4)
100–999 (5)
1,000–9,999 (1)

Projects / Programmes source: ARIS

source: ARIS

Molekularna biologija v biotehnološih raziskavah nižjih evkariontov (Slovene)

Research activity

Code	Science	Field	Subfield
4.06.01	Biotechnical sciences	Biotechnology	Recombinant DNA technology

Code	Science	Field
T000	Technological sciences
T490	Technological sciences	Biotechnology

Keywords

Saccharomyces cerevisiae, CDC6, cell cycle, two hybrid system; Aspergillus niger, filamentous fungi, hTNF-alpha, hG-CSF, EK, heterologous gene expression, protein secretion; Cochliobolus lunatus, Rhizopus nigricans, steroid monooxygenase, xenobiotics, plant steroids.

Evaluation (rules)

source: COBISS

Evaluation of bibliographic research performance indicators according to ARIS methodology

Citations Citations for bibliographic records in COBIB.SI that are linked to records in citation databases

source: WoS

source: Scopus

source: COBISS

Researchers (12)

no.	Code	Name and surname	Research area	Role	Period	No. of publicationsNo. of publications
1.	16104	PhD Apolonija Bedina Zavec	Biotechnology	Researcher	1999 - 2001	152
2.	06457	PhD Aleksandra Comino	Biochemistry and molecular biology	Researcher	1999 - 2001	61
3.	06108	PhD Vladimira Gaberc-Porekar	Biotechnology	Head	1999 - 2001	220
4.	18672	Mateja Jeras	Biochemistry and molecular biology	Researcher	2000 - 2001	25
5.	17275	Ana Marija Jesenko		Researcher	1999 - 2001	0
6.	06135	PhD Radovan Komel	Biochemistry and molecular biology	Researcher	1999 - 2001	1,054
7.	00385	PhD Nada Kraševec	Biotechnology	Researcher	1999 - 2001	214
8.	17276	Jelka Lenarčič		Researcher	1999 - 2001	2
9.	16105	MSc Irena Lukančič	Biotechnology	Researcher	1999 - 2001	11
10.	06707	PhD Aleksander Pavko	Biotechnology	Researcher	1999 - 2001	339
11.	12200	PhD Marko Vitas	Biochemistry and molecular biology	Researcher	1999 - 2001	77
12.	11250	PhD Polona Žnidaršič Plazl	Biotechnology	Researcher	1999 - 2001	446

Organisations (3)

no.	Code	Research organisation	City	Registration number	No. of publicationsNo. of publications
1.	0104	National Institute of Chemistry	Ljubljana	5051592000	20,982
2.	0103	University of Ljubljana, Faculty of Chemistry and Chemical Technology	Ljubljana	1626990	23,092
3.	0381	University of Ljubljana, Faculty of Medicine	Ljubljana	1627066	48,236

Abstract

The project is composed of three parts: A/ Interactions of Cdc6 protein in the cell cycle of yeast S.cerevisiae: B/ Cloning, expression and secretion of heterologous proteins in the filamentous fungus A.niger; C/ Some aspects of steroid metabolism in filamentous fungi. A/By the “Interaction trap” method, using CDC6 gene product as a bait, four gene products interacting with Cdc6 protein were detected. Most of them are genes with unknown function and none of them has been described to interact with CDC6 yet. Our research will focus on elucidation of interactions of these genes with CDC6, one by one and within the yeast cell cycle. B/ We have already expressed hTNF-alpha gene in A. niger but without secretion into the culture broth. At the same time, preliminary results were obtained on expression and secretion of two other proteins, hG-CSF and bEK. The aim of this research proposal is to apply scientific methods to improve production and secretion of these recombinant proteins for biotechnological application, and to investigate the reasons for impaired processing and secretion of recombinant hTNF-alpha - as a model for research on heterologous protein secretion mechanisms in filamentous fungi. C/ By induction experiments, involvement of fungal steroid monooxygenases in their protecting role against plant steroids and some xenobiotics will be studied. We will also try to clone some monooxygenases by PCR and RACE-PCR approach.