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Projects / Programmes source: ARIS

Nevroendokrino uravnavanje tonusa koronarnega žilja (Slovene)

Research activity

Code Science Field Subfield
3.03.00  Medical sciences  Neurobiology   

Code Science Field
B640  Biomedical sciences  Neurology, neuropsychology, neurophysiology 
B530  Biomedical sciences  Cardiovascular system 
Keywords
coronary vascular tonus, in vivo stimulation of vegetative nervus system, isolated coronaries, endothelium cell cultures, actinoporins, dynamics of intracellular calcium activity, endothelium released mediators, endothelin I
Evaluation (rules)
source: COBISS
Researchers (4)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  15667  PhD Matjaž Bunc  Neurobiology  Head  2000 - 2002  530 
2.  04293  PhD Janez Rozman  Systems and cybernetics  Researcher  2000 - 2002  245 
3.  09861  Jerneja Strupi-Šuput  Civil engineering  Researcher  2000 - 2002  134 
4.  07002  PhD Dušan Šuput  Neurobiology  Researcher  2000 - 2002  433 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  48,182 
2.  1326  ITIS, Implantable Technology and Sensors, producing, trading, servicing and researching enterprise, d.o.o. Ljubljana  Ljubljana  5824796000  245 
Abstract
Atherosclerosis is the main cause of myocardial ischemic events. Less often coronary vasospasm could be also the cause of myocardial ischemia but the mechanism is still obscure. We suppose that endothelial dysfunction should be involved in the mechanism of coronary vasospasm, but the importance of vegetative nervous system is unknown. We are going to provide stimulation of both parasympathic and sympathic nerve system in a dog animal model. The main goal of our experiments is to find out the influence of chronic intermitent stimulation of stellat ganglions and vagal nerve on morfologic changes of coronary arteries and appearance of coronary vasospasm. Interactions between endothelium and vascular smooth muscle will be studied on rings of isolated pig coronaries. Equinatoxin II, actinoporin isolated from the sea anemone Acinia equina (L.), will be used as a kind of biological tool for the releasing of vasoactive substances from endothelium. Through the modulation of the effects of released mediators by different blocker of the endotehlial function (methylen blue, indomethacin) and receptors on vascular smooth muscle (calcium channel blocker type L, endothelin receptorsblockers), the mechanism of contraction caused by equinatoxin II will be studied. Equinatoxin II will be used as biological tool for paracrine releasing of endotelin I. The last mentioned effect of equinatoxin II will be used for testing of potency of different available blockers of endotelin receptors. Intracellular activity of calcium ions and morphologic changes of endotehlial cell cultures due to the toxin action will be studied too. Simultaneouslly with the measurements of intracellular calcium activity we are going to measure releasing of NO, endothelin I and products of arachidonic acid metabolism.
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