Projects / Programmes
Deciphering redox-related signaling interconnectedness in potato resistance against viruses
| Code |
Science |
Field |
Subfield |
| 4.06.00 |
Biotechnical sciences |
Biotechnology |
|
| Code |
Science |
Field |
| 4.04 |
Agricultural and Veterinary Sciences |
Agricultural biotechnology |
Solanum tuberosum (potato), potato virus Y, hypersensitive response-conferred resistance, programmed cell death, organelle specific ROS, salicylic acid, stromules, spatiotemporal transcriptomics, confocal microscopy, redox state, NADPH oxidase RBOHD
Data for the last 5 years (citations for the last 10 years) on
May 6, 2024;
A3 for period
2018-2022
Data for ARIS tenders (
04.04.2019 – Programme tender,
archive
)
| Database |
Linked records |
Citations |
Pure citations |
Average pure citations |
| WoS |
13 |
118 |
98 |
7.54 |
| Scopus |
15 |
139 |
116 |
7.73 |
Researchers (1)
| no. |
Code |
Name and surname |
Research area |
Role |
Period |
No. of publicationsNo. of publications |
| 1. |
36323 |
PhD Tjaša Lukan |
Biology |
Head |
2021 - 2024 |
125 |
Organisations (1)
| no. |
Code |
Research organisation |
City |
Registration number |
No. of publicationsNo. of publications |
| 1. |
0105 |
National Institute of Biology |
Ljubljana |
5055784 |
13,297 |
Abstract
Understanding the interaction between plants and pathogens is crucial to ensure long lasting and environmentally friendly system for plant protection. Potato virus Y (PVY) is one of the most detrimental pathogens of cultivated potatoes, causing significant yield losses worldwide. As a response to PVY infection, potato cv. Rywal develops Ny-1-mediated hypersensitive response (HR)-conferred resistance, which is accompanied by the induction of HR programmed cell death on inoculated leaves. The HR-conferred resistance to PVY has been intensively studied, yet the downstream signaling mechanisms leading to restriction of pathogen spread remain mostly unknown. Results of our recent studies indicate on the coordinated roles of apoplastically generated reactive oxygen species (apoROS), chloroplastic ROS (chlROS), salicylic acid (SA) signaling and stromule formation in signaling for HR-conferred resistance. We hypothesise that only a precisely temporally and spatially coordinated and intertwined actions of all key players enable effective immune response. To address the dynamic and interconnected nature of this response, an innovative and interdisciplinary approach is required. Therefore, we will combine synthetic biology, in planta functional studies, transcriptomics analyses and in vivo imaging with high spatiotemporal resolution to explore the roles of key players in HR-conferred resistance in potato. We will produce chloroplast redox state sensor plants with perturbed chlROS, apoROS and SA abundance in different combinations, which will allow us to decipher interconnectedness between them and to identify new potential key properties of signaling network. The proposed project will provide a breakthrough in understanding the signaling mechanisms of viral resistance. In addition, the valuable research tools developed for monitoring the effect of different key components will lay a foundation for understanding the signaling mechanisms of plant defense response also in other pathosystems.
