Projects / Programmes
Imunoloski vidiki presaditve tankega crevesa in drugih organov (ledvica, trebusna slinavka) (Slovene)
| Code |
Science |
Field |
Subfield |
| 3.06.00 |
Medical sciences |
Cardiovascular system |
|
small intestinal, kidney and pancreas transplantation, apoptosis, graft versus host disease, inflamation, proliferative response
Researchers (3)
| no. |
Code |
Name and surname |
Research area |
Role |
Period |
No. of publicationsNo. of publications |
| 1. |
10614 |
PhD Mirko Omejc |
Oncology |
Researcher |
2000 - 2002 |
259 |
| 2. |
16247 |
PhD Tadeja Pintar |
Cardiovascular system |
Researcher |
2000 - 2002 |
320 |
| 3. |
10909 |
PhD Alojz Pleskovič |
Neurobiology |
Head |
2000 - 2002 |
197 |
Organisations (1)
Abstract
Objective.The combination of bone marrow transplant together with solid organ transplantation has been suggested to augment chimerism and facilitate the development of donor specific tolerance.The grade of graft-versus-host disease is supposed to depend on the dinamic process of immune response activation within the recipient. By the use of different immunosuppressive substances it is possible to interfere in the two-way dynamic process between the recipient of the solid organ and transplanted organ.
Immunohistological stainning of the transplanted organ and skin biopsies would be helpful in estimating the type of cellular infiltrates that could explain immunological events after combined bone marrow, small intestinal transplantation and pancreas and /or kidney.
Methods
We itend to do combined smal intestinal, pancreatic or kidney and bone marrow transplantation from the same donor in pig experimental model. Immunossuppressive treatment with rapamycin, FK 506 and /or mycophenolate mofetil will be used in the defined experimental group based on the experimental protocol.
The purpose of the study is to declare the reason of different small intestinal (grft) and pancreatic or kidney comparing skin graft versus host disease in small bowel, pancreas or kidney and bone marrow cells recipient.
To succeed histological sings of GVHD we intend to use the same protocol on pig experimental model already used in preceding experimental work.
To explain immunological events following solid organ and bone marrow transplantation we intend to use the next histological staining of the intestinal mucosa of the graft, pancreatic cells or kidney and skin biopsies. Following fixation we will use:
1. HE (hemetoxilin-eosin) staining
2. SAB stainning to present the inflamation parameters (tissue basofilis infiltration)
3. TUNEL,p53, BCL2,MIB: to evaluate apoptotic events in the transplanted organ and skin of the recipient
4.PCNA stainning to evaluate proliferative response after solid organ and bone marrow cells transplantation
5. Scanning miscoscopy
Based on half resoults of an experimental work new additional procedures will be used, if any need.
A very important ascertainment that follows the preceeding experimental work is that bone marrow transplantation provokes different kinds of graft versus host disease in the transplanted organ and skin of the recipient. With the specific use of already mentined histological technics we will evaluate the type of cellular infiltrates in the skin of recipient and explain differences that are specific for particular organ and bone marrow transplantation.
