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Projects / Programmes source: ARRS

Mikrobiologija in mikrobna biotehnologija (Slovene)

Periods
January 1, 1999 - December 31, 2003
Research activity

Code Science Field Subfield
4.02.00  Biotechnical sciences  Animal production   
4.06.00  Biotechnical sciences  Biotechnology   

Code Science Field
B230  Biomedical sciences  Microbiology, bacteriology, virology, mycology 
B420  Biomedical sciences  Nutrition 
B400  Biomedical sciences  Zootechny, animal husbandry, breeding 
T490  Technological sciences  Biotechnology 
Evaluation (rules)
source: COBISS
Researchers (11)
no. Code Name and surname Research area Role Period No. of publications
1.  19485  PhD Tomaž Accetto  Animal production  Researcher  2002 - 2003  159 
2.  08320  PhD Gorazd Avguštin  Animal production  Researcher  2002 - 2003  351 
3.  21437  PhD Tadej Čepeljnik  Biotechnology  Researcher  2002 - 2003  51 
4.  11765  PhD Lijana Fanedl  Animal production  Researcher  2002 - 2003  98 
5.  23439  PhD Gregor Gorenc  Animal production  Researcher  2003 
6.  17528  Marta Majdič    Researcher  2002 - 2003 
7.  08404  PhD Romana Marinšek Logar  Animal production  Researcher  2002 - 2003  501 
8.  03098  PhD Franc-Viktor Nekrep  Animal production  Principal Researcher  2002 - 2003  196 
9.  16327  PhD Matjaž Peterka  Biotechnology  Researcher  2002 - 2003  210 
10.  16322  MSc Katarina Tepšič  Animal production  Researcher  2002 - 2003  22 
11.  17864  PhD Maša Zorec  Animal production  Researcher  2002 - 2003  68 
Organisations (1)
no. Code Research organisation City Registration number No. of publications
1.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  64,118 
Abstract
Molecular biology & genetics offered new ways into investigation of complex microbial ecosystems. The core of the programme deals with the microbial community inhabiting the animal gastrointestinal tracts, predominantly rumen. The community structure and dynamics are investigated by means of molecular approaches i.e. direct cloning of the 16S rRNA genes and subsequent phylogenetic analysis and the design, production and use of specific oligonucleotide probes in PCR and in situ hybridisation experiments followed by epifluorescent microscopy or flow cytometry detection and analysis. Ribosomal genes and whole operons are being cloned from predominant rumen bacterial species and sequence, structure and function analysed are performed. The main interest is focused into the analysis of the dominant rumen bacterial genera i.e. Prevotella and Butyrivibrio, however, other genera from non ruminal environment are investigated too. Molecular tools for the detection and monitoring of salmonella and bifidobacteria are being developed and the total bacterial community inhabiting the terestrial isopode crustacean Porcelio scaber is being investigated. The impact of antinutrive agents i.e. plant lectins on microbial flora inhabiting the animal small intestine is being investigated. Genetic and biochemical analyses of enzymatic activities of ruminal microorganisms are being performed too. Nuclease genes from ruminal prevotellas are being cloned and their activity enzymaticalla analysed. The xylanase genes of ruminal butyrivibrios have been shown to belong to the most potent polysaccharidases of ruminal origin and the biotechnological potential is being investigated as well as prebiotic and probiotic activities of a new Butyrivibrio isolate. Other ecological niches are being investigated in collaboration with the different research group. The molecular tools are being developed for the Pseudomands inhabiting soil and phytopatogenic bacteria (Ralstonia, Erwinia etc.) in colaboration with the Planta center of the national Institute for Biology.
Most important scientific results Final report
Most important socioeconomically and culturally relevant results Final report
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