Loading...
Projects / Programmes source: ARIS

Enzymology in Signal Transduction

Periods
Research activity

Code Science Field Subfield
3.03.00  Medical sciences  Neurobiology   

Code Science Field
P004  Natural sciences and mathematics  Biochemistry, Metabolism 
P310  Natural sciences and mathematics  Proteins, enzymology 
B640  Biomedical sciences  Neurology, neuropsychology, neurophysiology 
Evaluation (rules)
source: COBISS
Researchers (7)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  16130  PhD Aljoša Bavec  Neurobiology  Researcher  2001 - 2003  130 
2.  13784  PhD Branko Ermenc  Stomatology  Researcher  2001 - 2003  85 
3.  19129  PhD Marko Goličnik  Neurobiology  Researcher  2001 - 2003  144 
4.  17883  Nevenka Klenovšek Špat    Researcher  2001 - 2003 
5.  17884  Klavdija Makovec    Researcher  2001 - 2003 
6.  03723  PhD Jurij Stojan  Neurobiology  Researcher  2001 - 2003  264 
7.  04350  PhD Matjaž Zorko  Neurobiology  Head  2001 - 2003  190 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  48,446 
Abstract
Program represents fundamental research in the field of protein neurochemistry and is based on the studies of protein - protein as well as protein – small molecule(s) intramolecular interactions. Different cholinesterases and G-proteins are used as model systems. We try to correlate mathematical models of molecular interactions with molecular visualization of the active and regulatory sites of studied proteins, using methods of enzyme kinetics and thermodynamics, purification and identification of proteins, synthesis of model peptides, and molecular modeling. As we hope, our results will contribute to general understanding of the role of cholinesterases in synaptic transmission and clarification of signal transduction mechanisms via G-protein cascades. More specifically, our research should contribute to a design of better active substances for the treatment of signal translation related pathological situations, for instance Alzheimer’s disease, as well as to a design of more selective insecticides. Additionally, we collaborate in development of peptide vector molecules for the transfer of biologically active substances across the biological membranes and blood-brain barrier. In the field of cholinesterases we try to establish a general model of the molecular mechanism for the homo- and heterotropic substrate cooperativity, valid for non-vertebrate (Drosophila) and vertebrate (eel, ray, horse, and mouse) cholinesterases. In this studies we use active site and peripheral site directed ligands, as well as isolated wild type, recombinant and site directed mutated enzymes. In the field of G-proteins we wish to contribute in clarification of molecular background of G-proteins coupling to different receptors and interacting with different physiologically important peptides, for instance ?-amyloid peptide and potential drug translocating peptides. In these studies we use synthetic and natural peptides including with some site specific mutated peptides. Membranes isolated from different brain regions and cell lines, as well as isolated and recombinant G-proteins of different types will be used.
Most important scientific results Final report
Most important socioeconomically and culturally relevant results Final report
Views history
Favourite