Projects / Programmes source: ARIS

Genska tehnologija za fermentacijsko proizvodnjo biološko aktivnih beljakovin (Slovene)

January 1, 1999 - December 31, 2003
Research activity

Code Science Field Subfield
4.06.00  Biotechnical sciences  Biotechnology   

Code Science Field
P320  Natural sciences and mathematics  Nucleic acids, protein synthesis 
P310  Natural sciences and mathematics  Proteins, enzymology 
T490  Technological sciences  Biotechnology 
Evaluation (rules)
source: COBISS
Researchers (18)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  16104  PhD Apolonija Bedina Zavec  Biotechnology  Researcher  2001 - 2003  143 
2.  06457  PhD Aleksandra Comino  Biochemistry and molecular biology  Researcher  2001 - 2003  61 
3.  20381  PhD Irena Fonda  Biochemistry and molecular biology  Researcher  2001 - 2003  43 
4.  06108  PhD Vladimira Gaberc-Porekar  Biotechnology  Head  2001 - 2003  220 
5.  18672  Mateja Jeras  Biochemistry and molecular biology  Researcher  2001 - 2003  25 
6.  17275  Ana Marija Jesenko    Researcher  2001 - 2003 
7.  06135  PhD Radovan Komel  Biochemistry and molecular biology  Researcher  2001 - 2003  1,053 
8.  00385  PhD Nada Kraševec  Biotechnology  Researcher  2001 - 2003  208 
9.  23473  PhD Ljerka Lah  Biotechnology  Researcher  2003  60 
10.  17277  Zdenka Laznik    Researcher  2001 - 2002 
11.  17276  Jelka Lenarčič    Researcher  2001 - 2003 
12.  21241  PhD Ana Lenassi Zupan  Biotechnology  Researcher  2001 - 2003  16 
13.  18210  Nataša Lileg-Tašler    Researcher  2001 - 2003 
14.  16105  MSc Irena Lukančič  Biotechnology  Researcher  2001 - 2002  11 
15.  22581  PhD Mateja Novak Štagoj  Biotechnology  Researcher  2002 - 2003  20 
16.  22580  PhD Špela Peternel  Biochemistry and molecular biology  Researcher  2002 - 2003  42 
17.  21684  Tea Tomšič    Researcher  2002 - 2003 
18.  09178  PhD Janko Žmitek  Pharmacy  Researcher  2003  296 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0104  National Institute of Chemistry  Ljubljana  5051592000  21,377 
The proposed research program is based on the long-term experience in biochemical, bio-molecular and biotechnological research of lower eucariotes, especially filamentous fungi. In the seventies and eighties the research group took part in the development of the submerged production of ergot alkaloids by Claviceps fungi, which was transferred to production in the Pharmaceutical and Chemical Company Lek. In the nineties, the group extended its research area to genetic and protein engineering for biosynthesis of biologically active and pharmacologically useful recombinant proteins. Using human tumour necrosis factor alpha (TNF) as a model protein, they succeeded to design, clone and express in E. coli numerous new analogues with newly introduced properties. Many of them have been pre-clinically tested at the Institute of Oncology in Ljubljana, and one of them showing significantly improved therapeutic properties seems to be an excellent candidate for future clinical testing. Lower eucariotes (yeast, filamentous fungi) exhibit numerous advantages for expression of heterologous recombinant proteins, such as high production, ability of post-translational modifications, GRAS status, secretion into the medium, which results in simpler purification, etc. Therefore, the scope of this research program is development of procedures for biosynthesis of highly purified recombinant proteins in the lower eucariote expression-secretion systems. This includes a whole series of research methodologies: from studies of biochemistry and molecular biology of some basic cell life properties of lower eucariotes, design of gene constructs and their synthesis by PCR, site directed mutagenesis, cloning in E. coli, expression of heterologous gene constructs in yeast and filamentous fungi, research of processing and secretion mechanisms, to research of optimisation and scale-up of fermentation, studies on isolation and purification procedures and testing of biological properties of recombinant products. Some basic research will be done on proteins involved in the cell cycle of S. cerevisiae. Computer modelling and relevant r-DNA technologies will be used for elaboration of gene constructs for TNF, G-CSF, EK, GFP, some enzymes of steroid metabolism and respective analogues, which will be expressed in yeast (e.g. P. pastoris) and filamentous fungi (e.g. A. niger) and used as model molecules for studies of secretion mechanisms. Protein engineering will also be applied for research of optimised purification methodologies using engineered purification handles. "In vitro" biological testing of recombinant proteins will be done on mammalian cell cultures, while "in vivo" biological testing of their pharmacological and therapeutic properties will be performed in collaborative institutions.
Most important scientific results Final report
Most important socioeconomically and culturally relevant results Final report
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