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Projects / Programmes source: ARIS

Physiology, biochemistry and molecular biology of micro-organisms

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Periods
January 1, 1999 - December 31, 2003
Research activity

Code Science Field Subfield
4.06.00  Biotechnical sciences  Biotechnology   
1.05.00  Natural sciences and mathematics  Biochemistry and molecular biology   

Code Science Field
P004  Natural sciences and mathematics  Biochemistry, Metabolism 
B230  Biomedical sciences  Microbiology, bacteriology, virology, mycology 
Evaluation (rules)
source: COBISS
Evaluation of bibliographic research performance indicators according to ARIS methodology
Citations Citations for bibliographic records in COBIB.SI that are linked to records in citation databases
source: WoS source: Scopus source: COBISS
Researchers (6)
no. Code Name and surname Research area Role Period No. of publications
1.  14360  PhD Mojca Benčina  Biotechnology  Researcher  2001 - 2003  392 
2.  06109  PhD Katarina Jernejc  Biotechnology  Researcher  2001 - 2003  107 
3.  19362  MSc Nataša Labanac  Biochemistry and molecular biology  Researcher  2001 - 2002  5 
4.  09354  PhD Matic Legiša  Biotechnology  Head  2001 - 2003  272 
5.  17280  Darija Oven    Researcher  2001 - 2003  4 
6.  17281  Irena Škraba    Researcher  2001 - 2003  0 
Organisations (1)
no. Code Research organisation City Registration number No. of publications
1.  0104  National Institute of Chemistry  Ljubljana  5051592000  21,007 
Abstract
Slovenian biotechnological industry has a tradition of several decades and the development of basic processes for bioproduct production is well established. However, due to extremely rapid development of new methods in modern biotechnology, i.e. recombinant DNA technology, the people from the industries in great extend lack the knowledge and technology for manipulating productive micro-organisms . Among others not enough attention was paid for the studies of physiology, biochemistry and molecular biology of industrial micro-organisms and the ability to improve these organisms by means of recombinant DNA technology. At the National Institute of Chemistry a number of modern methods of molecular biology has been adopted recently which are indispensable for the studies of functioning of microbial cells. Step by step the methods such as determination of intracellular intermediates, enzyme kinetics, isolation and characterisation of enzymes, measuring intracellular pH, the use of radioactively labels molecules for analytical purposes, methods of recombinant DNA technology were introduced into the labs. Within the research programme we will use the modern methodology to study the response of microbial cells to sudden changes in the environment. First of all a signal transduction mediated by cyclic AMP as a secondary messenger will be investigated in more details followed by thorough analysis of signalling via lipid mediators such as inositol-phosphates and diacylglycerol. The effect of Ca2+ ions release from the intracellular reserves on primary metabolism will be studied as well. The genes for the key enzymes involved in synthesis and degradation of secondary messenger molecules will be cloned and sequenced. Transformants with silenced and overexpressed genes will be isolated that will help us to understand the physiological role of specific signalling pathways in regulation of metabolism. The activities of protein kinases will be studied and the target proteins determined. However, the main goal of research within the programme is to adopt the modern methods of molecular biology and biochemical analytics by which we can conduct the research on any other micro-organism on request from the industries.
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