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Projects / Programmes source: ARIS

Molekularna nevrobiologija (Slovene)

Periods
January 1, 1999 - December 31, 2003
Research activity

Code Science Field Subfield
3.03.00  Medical sciences  Neurobiology   

Code Science Field
P320  Natural sciences and mathematics  Nucleic acids, protein synthesis 
B450  Biomedical sciences  Development biology, teratology, ontogeny, embryology (human) 
B640  Biomedical sciences  Neurology, neuropsychology, neurophysiology 
Evaluation (rules)
source: COBISS
Researchers (6)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  11903  PhD Martina Brank  Biotechnology  Researcher  2001 - 2003  28 
2.  07089  Zvonka Frelih    Researcher  2001 - 2003 
3.  04410  PhD Zoran Grubič  Neurobiology  Head  2001 - 2003  384 
4.  16345  PhD Tomaž Marš  Neurobiology  Researcher  2001 - 2003  344 
5.  19318  PhD Katarina Miš  Neurobiology  Researcher  2001 - 2003  191 
6.  05046  PhD Majda Zorec-Karlovšek  Neurobiology  Researcher  2001 - 2002  290 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  46,268 
Abstract
Our research field are the molecular mechanisms underlying synaptogenesis and plasticity of the mammalian neuromuscular junction (NMJ). Basic events of the cholinergic neuromusular transmission have already been established, however, our present understanding of other aspects of neuromuscular communications, especially of the mechanisms controlling and regulating expression of synaptic components and their targeting to the sites of their action, is still fragmentary. These mechanisms are the basis of synaptic formation and plasticity not only in the NMJ but, presumably, also in other types of synapses and are therefore essential for several functions of the nervous system. Our approach at the investigations of these mechanisms is based on examination of synaptic protein components at different levels of their synthesis (mainly at the mRNA level and mature protein level.) under different experimental conditions. The NMJ component most frequently studied in our experiments so far has been acetylcholinesterase. Recently, we extended our investigations on the effects of agrin on the functional maturation of NMJ in the cultured human muscle. Experimental models employed in our laboratory include normal, glucocorticoid-treated and denervated adult rat skeletal muscle, adult rat spinal cord and in vitro innervated human muscle. To follow localization of NMJ components we use nonradioactive in situ hybridization, immunocytochemistry and standard histochemical techniques combined with classical and confocal microscopy. For quantitative determinations of mRNAs we use RNA blot hybridization and recently introduced competitive RT PCR. Western blot and radiometric techniques are used for quantitative determinations of mature proteins.
Most important scientific results Final report
Most important socioeconomically and culturally relevant results Final report
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