Projects / Programmes
Molekularna nevrobiologija (Slovene)
January 1, 1999
- December 31, 2003
Code |
Science |
Field |
Subfield |
3.03.00 |
Medical sciences |
Neurobiology |
|
Code |
Science |
Field |
P320 |
Natural sciences and mathematics |
Nucleic acids, protein synthesis |
B450 |
Biomedical sciences |
Development biology, teratology, ontogeny, embryology (human) |
B640 |
Biomedical sciences |
Neurology, neuropsychology, neurophysiology |
Researchers (6)
Organisations (1)
Abstract
Our research field are the molecular mechanisms underlying synaptogenesis and plasticity of the mammalian neuromuscular junction (NMJ). Basic events of the cholinergic neuromusular transmission have already been established, however, our present understanding of other aspects of neuromuscular communications, especially of the mechanisms controlling and regulating expression of synaptic components and their targeting to the sites of their action, is still fragmentary. These mechanisms are the basis of synaptic formation and plasticity not only in the NMJ but, presumably, also in other types of synapses and are therefore essential for several functions of the nervous system.
Our approach at the investigations of these mechanisms is based on examination of synaptic protein components at different levels of their synthesis (mainly at the mRNA level and mature protein level.) under different experimental conditions. The NMJ component most frequently studied in our experiments so far has been acetylcholinesterase. Recently, we extended our investigations on the effects of agrin on the functional maturation of NMJ in the cultured human muscle. Experimental models employed in our laboratory include normal, glucocorticoid-treated and denervated adult rat skeletal muscle, adult rat spinal cord and in vitro innervated human muscle. To follow localization of NMJ components we use nonradioactive in situ hybridization, immunocytochemistry and standard histochemical techniques combined with classical and confocal microscopy. For quantitative determinations of mRNAs we use RNA blot hybridization and recently introduced competitive RT PCR. Western blot and radiometric techniques are used for quantitative determinations of mature proteins.
Most important scientific results
Final report
Most important socioeconomically and culturally relevant results
Final report