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Projects / Programmes source: ARIS

Biochemical studies of the membrane-active protein from the mushroom Pleurotus ostreatus

Research activity

Code Science Field Subfield
1.05.00  Natural sciences and mathematics  Biochemistry and molecular biology   

Code Science Field
P004  Natural sciences and mathematics  Biochemistry, Metabolism 
Keywords
hemolytic protein, oyster mushroom, Pleurotus ostreatus, pore-forming protein, toxin, initiation, biotechnology
Evaluation (rules)
source: COBISS
Researchers (4)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  06994  PhD Peter Maček  Biochemistry and molecular biology  Researcher  2002 - 2004  523 
2.  17422  Irena Pavešič    Researcher  2002 - 2004 
3.  15328  PhD Kristina Sepčić  Biochemistry and molecular biology  Head  2002 - 2004  731 
4.  06905  PhD Tom Turk  Biochemistry and molecular biology  Researcher  2002 - 2004  620 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  66,832 
Abstract
A new hemolytic protein was isolated from the edible oyster mushroom (Pleurotus ostreatus). The molecule has been preliminarly characterized. Results have shown that its N-terminal amino acid sequence possesses high homology with some other known fungal proteins with marked biological activities. One of them, Asp-hemolysin from Aspergillus fumigatus, shows high binding affinity to low density plasma lipoprotein (LDL) and other, derived from cDNA sequence of Agrocybe aegerita, is involved in fruiting initiation of the mushroom. These biological effects could lead to a potential use of our protein in the fields of biotechnology and pharmacy. Within the proposed project, we would like to optimize the purification procedure of the hemolytic protein from Pleurotus ostreatus, and to determine its biochemical and physical properties. Furthermore, we would like to study the interaction of the protein with natural and artificial biological membranes in details. The interaction of the protein with LDL and its effect on fruiting initiation will be also tested. The protein will be molecularly cloned and expressesed in a heterologous system by using molecular biology techniques.
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