Projects / Programmes
Peripheral autonomic nerves: Stimulation and sensing
| Code |
Science |
Field |
Subfield |
| 2.06.07 |
Engineering sciences and technologies |
Systems and cybernetics |
Biomedical technics |
| Code |
Science |
Field |
| B115 |
Biomedical sciences |
Biomechanics, cybernetics |
Electrical stimulation, implantable electrode systems, peripheral autonomic nerves, pancreas
Researchers (1)
| no. |
Code |
Name and surname |
Research area |
Role |
Period |
No. of publicationsNo. of publications |
| 1. |
04293 |
PhD Janez Rozman |
Systems and cybernetics |
Head |
2002 - 2004 |
245 |
Organisations (1)
Abstract
The hypothesis is that with selective stimulation of certain superficial regions of peripheral nerves of autonomic neural system, innervating the pancreas of a dog it is possible to increase secretion of the hormones insulin and glucagon in different mutual ratios into the blood in healthy animal and in animal with diabetes type I or type II where certain portion of islets of Langerhans remained in function. The hypothesis is also that it is possible with the same chronically implanted multielectode spiral cuff systems to selectively record electrical activity from certain superficial regions of aforementioned nerves and to analyse it as an information potentially used in neurological evaluation of the status of a diabetes and also to analyse it as an information needed in closed loop control of multi-channel implantable systems.
According to the model described in the proposal and the aim of testing the hypothesis two healthy Beagle dogs weighing about 15kg will be used as experimental animals. Six 33-electrode spiral cuff sistems will be fabricated and implanted on the vagus nerve, the splanchinc nerve and the pancreatic nerve of both experimental animals.
In the first series of animal experiments changes in secretion of insulin, glucagon and C-peptide hormones from the pancreas as well as the level of glucose in the blood will be tested as a consequence of selective stimulation of certain superficial regions of aforementioned nerves in healthy animals. Then, selective recording of superficial activity from the same superficial regions of the nerves will be recorded.
In the second series of animal experiments changes in secretion of aforementioned hormones will be tested at the same conditions but in animals with dibetes caused by administration of Alloxan.
Before and after each stimulation sequence of aforementioned nerves the blood samples from the femoral artery and jugular vein will be drained out and stored untill radioimmunological assay. In all samples the quantity of hormones insulin, glucagon and C-peptide will be determined. At the end all results regarding radioimmunological assay as well as results regarding selective recording will be analyzed and presented.
