Projects / Programmes
Analysis of gene expression associated with apoptosis using microarrays
| Code |
Science |
Field |
Subfield |
| 3.04.00 |
Medical sciences |
Oncology |
|
| Code |
Science |
Field |
| B740 |
Biomedical sciences |
Pharmacological sciences, pharmacognosy, pharmacy, toxicology |
pharamcogenomics, BCR, gene expression , apoptosis, microarray
Researchers (4)
Organisations (2)
Abstract
Apoptotic cell death is a critical event in tumorogenesis, immune dysfunctions and drug-resistance. Understanding the molecular events that contribute to the induction of apoptosis, and prediction of possible modulation shall enable a more rational approach to drug design and therapy.
With the imminent completion of the Human Genome Project, biomedical research gained the ability to carry out investigations on a genome wide scale. Gene expression microarray technology is gaining increasingly widespread use as a means to determine the expression of potentially all human genes. It has been utilized as a means to determine new molecular targets for drug development, discovery of new diagnostic and prognostic indicators, biomarkers of therapeutic response, and others.
The purpose of our work is to gain better understanding of gene expression that contribute to B-cell receptor transduced apoptotic process. Proposed study is based on B-lymphoblastoid cell line, a broadly accepted model of B cell receptor-induced apoptosis, which represents the mechanism of clonal deletion of self-reactive B1 cells in vivo.
The first part of proposed research program has to deal with establishing appropriate apoptotic conditions. We next intend to utilize the microarray analysis, a highly efficient method of gene expression profiling, which shall provide insights into regulatory mechanisms and biochemical pathways of apoptosis. We shall conclude this project by identifying key regulatory elements of BCR-induced apoptosis. Identification of new molecules and mechanisms shall serve a basis of targeting intervention and modulation of a cell fate.
Proposed research is based on international collaboration of experts, ensuring high scientific relevance. Implementation of microarray technology shall enable its applications into other fields of pharmaceutical sciences and profession. This project provides the exchange of knowledge, availability of infrastructure, mobility of researcher and integration into European research area.
