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Projects / Programmes source: ARIS

Cytogenetic analysis of human oocytes and preimplantation embryos

Research activity

Code Science Field Subfield
3.05.00  Medical sciences  Human reproduction   

Code Science Field
B570  Biomedical sciences  Obstetrics, gynaecology, andrology, reproduction, sexuality 
Keywords
preimplantation genetic diagnosis, fluorescence in situ hybridization, oocytes, embryos
Evaluation (rules)
source: COBISS
Researchers (5)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  20134  PhD Mojca Čižek Sajko  Human reproduction  Researcher  2003 - 2005  110 
2.  13343  PhD Nadja Kokalj Vokač  Oncology  Researcher  2003 - 2005  450 
3.  11040  PhD Borut Kovačič  Human reproduction  Head  2003 - 2005  382 
4.  08996  PhD Veljko Vlaisavljević  Human reproduction  Researcher  2003 - 2005  594 
5.  15747  Andreja Zagorac  Human reproduction  Researcher  2003 - 2005  154 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0334  University Medical Centre Maribor  Maribor  5054150000  23,094 
Abstract
The goal of the project is to introduce the basic cytogenetic techniques, karyotyping and fluorescence in situ hybridization (FISH) on the single cell. The methods are introducing with the mean to omogočiti the preimplantation genetic diagnosis (PGD) on early preimplantation embryos, with which a specific chromosome linked genetic diseases could be diagnosed or prevented and chromosomal abnormalities of gametes and preimplantation embryos could be assessed. These abnormalities are the frequent cause of developmental disorders or developmental arrests of the embryos as well as the cause of chronic unseccessful in vitro fertilization treatments, which we were not able to detect to this moment. The method of single blastomere isolation from the embryo and the preparation of the slide, as well as staining and results evaluation need well trained team. The object for introducing of mentioned methods will be discarded unseccessfully and abnormally fertilized human oocytes and arrested preimplantation embryos from in vitro fertilization program. For fixation and chromosome staining a modified classic techniques for karyotypization will be used. X and Y chromosomes will be marked with the FISH method. Meiotic spindle microtubules of oocytes from different assisted reproduction techniques will be stained. We will asses how many cells is available for analysis. After that we will distinguish the percentage of oocytes and arrested embryos with chromosome anomalies and oocytes with disorders in meiotic spindle configuration, which cause numerical chromosome abnormalities.
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