Projects / Programmes
Cytogenetic analysis of human oocytes and preimplantation embryos
| Code |
Science |
Field |
Subfield |
| 3.05.00 |
Medical sciences |
Human reproduction |
|
| Code |
Science |
Field |
| B570 |
Biomedical sciences |
Obstetrics, gynaecology, andrology, reproduction, sexuality |
preimplantation genetic diagnosis, fluorescence in situ hybridization, oocytes, embryos
Researchers (5)
Organisations (1)
Abstract
The goal of the project is to introduce the basic cytogenetic techniques, karyotyping and fluorescence in situ hybridization (FISH) on the single cell. The methods are introducing with the mean to omogočiti the preimplantation genetic diagnosis (PGD) on early preimplantation embryos, with which a specific chromosome linked genetic diseases could be diagnosed or prevented and chromosomal abnormalities of gametes and preimplantation embryos could be assessed. These abnormalities are the frequent cause of developmental disorders or developmental arrests of the embryos as well as the cause of chronic unseccessful in vitro fertilization treatments, which we were not able to detect to this moment. The method of single blastomere isolation from the embryo and the preparation of the slide, as well as staining and results evaluation need well trained team. The object for introducing of mentioned methods will be discarded unseccessfully and abnormally fertilized human oocytes and arrested preimplantation embryos from in vitro fertilization program. For fixation and chromosome staining a modified classic techniques for karyotypization will be used. X and Y chromosomes will be marked with the FISH method. Meiotic spindle microtubules of oocytes from different assisted reproduction techniques will be stained. We will asses how many cells is available for analysis. After that we will distinguish the percentage of oocytes and arrested embryos with chromosome anomalies and oocytes with disorders in meiotic spindle configuration, which cause numerical chromosome abnormalities.
