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Projects / Programmes source: ARRS

Determination and assessment of dietary intake of various important fatty acids in human and animal nutrition

Research activity

Code Science Field Subfield
4.02.00  Biotechnical sciences  Animal production   

Code Science Field
B000  Biomedical sciences   
B006  Biomedical sciences  Agronomics 
B420  Biomedical sciences  Nutrition 
Keywords
fatty acids, methyl esters, unsaturated fatty acids, cis-fatty acids, trans-fatty acids, conjugated linoleic acid (CLA), izomer separation, gas chromatography, animal nutrition, human nutrition
Evaluation (rules)
source: COBISS
Researchers (2)
no. Code Name and surname Research area Role Period No. of publications
1.  15194  PhD Alenka Levart  Animal production  Researcher  2003 - 2004  309 
2.  00486  PhD Andrej Orešnik  Animal production  Principal Researcher  2003 - 2004  372 
Organisations (1)
no. Code Research organisation City Registration number No. of publications
1.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  64,093 
Abstract
Fatty acids are nutritional ambivalent, while some of them are undesired, even harmful for humans and animals, other proved to be health beneficiary even in very low amounts. The main aim of the proposed project is development and optimization of analytical procedures for the determination of fatty acid composition of different plant and animal biological samples with special emphasis on determination of positional in geometrical (cis/trans) isomers of various unsaturated fatty acids: C16:1, C18:1 and C18:2 (including and conjugated linoleic acid). The above-mentioned fatty acids are extremely important from physiological and nutritional point of view and have profound negative (C18:1t) or positive (CLA) effect on human and animal health. Collected data along with data on nutritional habits of Slovene population will enable us to estimate the nutritional intake of individual trans isomers of unsaturated fatty acids and conjugated linoleic acid. Accurate and precise determination of individual positional and geometrical isomers of unsaturated fatty acids in biological samples is very complex analytical task, due to large number of isomers, which have to be separated and due to very low concentration levels of analytes in the samples. Therefore we intend to intensively study possible interferences caused by inadequate sample preparation and complex sample matrices. For separation of fatty acids the most efficient chromatographic techniques will be used.
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