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Projects / Programmes source: ARIS

Biosignal transduction and membrane domain structure

Research activity

Code Science Field Subfield
1.02.00  Natural sciences and mathematics  Physics   

Code Science Field
B002  Biomedical sciences  Biophysics 
Keywords
biological membranes, domain structure, EPR, physical modeling, spin labels, glycocalyx
Evaluation (rules)
source: COBISS
Researchers (8)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  20208  PhD Zoran Arsov  Biotechnology  Researcher  2004 - 2007  135 
2.  21546  PhD Tilen Koklič  Physics  Researcher  2004 - 2007  138 
3.  08742  Marjanca Nemec    Technical associate  2004 - 2007  81 
4.  03070  PhD Slavko Pečar  Pharmacy  Researcher  2004 - 2007  415 
5.  00393  PhD Milan Valter Schara  Physics  Researcher  2004 - 2007  343 
6.  13005  PhD David Stopar  Plant production  Researcher  2004 - 2007  469 
7.  01119  PhD Marjeta Šentjurc  Biochemistry and molecular biology  Researcher  2004 - 2007  511 
8.  18273  PhD Janez Štrancar  Physics  Head  2004 - 2007  372 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0106  Jožef Stefan Institute  Ljubljana  5051606000  90,664 
2.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  66,279 
Abstract
The latest developments in biophysics suggest that biological membrane as heterogeneous systems composed of lipid bilayer, glycosylated surface (glycocalyx) and numerous active membrane proteins. The interactions between those building blocks enable mass transport as well as signal transduction across the membrane and probable also in the lateral direction. Despite numerous investigations membrane heterogeneities and their functions still remain unresolved, in particular the heterogeneity of glycocalyx and its interaction with lipid bilayer and membrane proteins. In this project the EPR spectroscopy together with spin labeling, special experimental approaches and modeling will be applied to explore the above-mentioned interactions and their influence on membrane heterogeneity. Either model membrane systems, which mimics the composition of the biological membrane of different cell types, or different cell lines will be studied. Further, the effect of various biologically active molecules, for example virus coat proteins, toxins and anesthetics, on membrane domain structure, will be investigated. The main focus will be the identification of interaction between different lateral domain structures (heterogeneities) lipid bilayer and glycocalyx which enables signal tranduction. This would change the general perception of biological membrane and its role in a signal transduction. Moreover, it could also open new possibilities in the development of lipid biosensors, for the detection of viruses (their coat proteins), various membrane proteins, toxins, anesthetics, etc.
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