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Projects / Programmes source: ARRS

Distribution, molecular evolution and adaptation of bacteria on animal hosts: chlamydiae and mycoplasmas

Research activity

Code Science Field Subfield
1.03.00  Natural sciences and mathematics  Biology   

Code Science Field
B230  Biomedical sciences  Microbiology, bacteriology, virology, mycology 
Keywords
bacteria, intracellular, chlamydia, mycoplasma, hosts, vertebrates, avertebrates, detection, monitoring, molecular methods, attachment
Evaluation (rules)
source: COBISS
Researchers (15)
no. Code Name and surname Research area Role Period No. of publications
1.  13654  PhD Jerneja Ambrožič Avguštin  Biochemistry and molecular biology  Researcher  2004 - 2007  233 
2.  08320  PhD Gorazd Avguštin  Animal production  Principal Researcher  2004 - 2007  351 
3.  07914  PhD Dušan Benčina  Veterinarian medicine  Researcher  2004 - 2007  223 
4.  21437  PhD Tadej Čepeljnik  Biotechnology  Researcher  2006 - 2007  51 
5.  11184  PhD Alenka Dovč  Veterinarian medicine  Researcher  2004 - 2007  452 
6.  05098  PhD Peter Dovč  Biotechnology  Researcher  2004 - 2007  906 
7.  10397  PhD Kazimir Drašlar  Biology  Researcher  2004 - 2007  154 
8.  11155  PhD Damjana Drobne  Biology  Mentor to junior researcher  2004 - 2007  819 
9.  11765  PhD Lijana Fanedl  Animal production  Researcher  2004 - 2007  98 
10.  18749  PhD Rok Kostanjšek  Biology  Researcher  2004 - 2007  448 
11.  24296  PhD Darja Kušar  Veterinarian medicine  Junior researcher  2004 - 2007  177 
12.  05008  PhD Mojca Narat  Biotechnology  Researcher  2004 - 2007  675 
13.  19104  PhD Blaž Stres  Animal production  Researcher  2004 - 2007  349 
14.  07737  PhD Jasna Štrus  Biology  Mentor to junior researcher  2004 - 2007  439 
15.  16065  PhD Primož Zidar  Biology  Researcher  2004 - 2007  166 
Organisations (2)
no. Code Research organisation City Registration number No. of publications
1.  0406  University of Ljubljana, Veterinary Faculty  Ljubljana  1627139  9,698 
2.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  64,093 
Abstract
Novel microbial symbionts belonging to the bacterial order Chlamydiales and Mycoplasmales were recently discovered and described from gastrointestinal tract of terrestrial isopod crustaceans. The preliminary investigations showed a much wider distribution and phylogenetic diversity of these microbes as it was believed till now. A considerable probability exists therefore, that these organisms or their still unknown relatives take part in symbiotic (positive or negative) interactions with other vertebrate or invertebrate hosts. A number of interesting and important questions comes forward in this regard, concerning the actual presence and distribution of these microbes, the nature of their evolution, which may be tightly connected to the evolution of their animal hosts, and least but not last the mutual effects which may manifest in morphological, physiological and/or genetic sense. The purpose of this project is thus to screen a number of potential vertebrate and invertebrate hosts with molecular methods e.g. in situ hybridization employing specific oligonucleotide probes and specific quali or quantitative chain polimerisation method (PCR) in order to see whether sequences from targeted microorganisms can be discovered in animal cells or tissue. The isolation and in vitro cultivation of the targeted microbes from positive hosts is going to be attempted subsequently. If this should prove impossible due to their symbiotic nature (as expected) a combination of molecular genetic, immunological and microscopic methods is going to be applied. We expect to determine successfully the phylogenetic taxonomic affiliation of these organisms, and develop new, more specific and successful molecular and immunological tools for better detection and monitoring of these microbes in their hosts and general environment. The nature of interactions is going to be investigated and the effects on the hosts and symbionts. We expect to discover several novel species, genera and possibly even families of these interesting and important microorganisms, to elucidate various aspects of these symbioses with different hosts and to explore the basics of the interaction in biochemical and genetic terms in model host organisms i.e. terrestrial isopod crustacean Porcellio scaber.
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