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Projects / Programmes source: ARIS

Colicins - model systems for studies of regulation of gene expression na dprotein-protein interactions

Research activity

Code Science Field Subfield
1.05.00  Natural sciences and mathematics  Biochemistry and molecular biology   

Code Science Field
B220  Biomedical sciences  Genetics, cytogenetics 
B230  Biomedical sciences  Microbiology, bacteriology, virology, mycology 
Keywords
Bacteriocins, colicins, Escherichia coli, regulation of gene expression, macromolecular evolution, plasmids, protein-protein interactions, membranes
Evaluation (rules)
source: COBISS
Researchers (7)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  15686  PhD Gregor Anderluh  Biochemistry and molecular biology  Researcher  2004 - 2007  980 
2.  00882  PhD Miklavž Grabnar  Biochemistry and molecular biology  Researcher  2004 - 2007  183 
3.  09836  PhD Blagajana Herzog-Velikonja  Biochemistry and molecular biology  Researcher  2004 - 2007  120 
4.  17420  Fani Oven    Technical associate  2004 - 2007  11 
5.  06902  PhD Zdravko Podlesek  Biochemistry and molecular biology  Researcher  2004 - 2007  137 
6.  16064  PhD Marjanca Starčič Erjavec  Biochemistry and molecular biology  Researcher  2004 - 2007  446 
7.  07042  PhD Darja Žgur Bertok  Biochemistry and molecular biology  Head  2004 - 2007  441 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  67,264 
Abstract
Colicins are bacteriocins synthesized by Escherichia coli strains. Synthesis and release of colicins is encoded by three genes: an activity gene, an immunity gene and a lysis gene which is responsible for colicin release through lysis of the producing cell. Colicins are important tools for studies of protein translocation through and into membranes and due to the large number of different colicins as a model of the evolution of macromolecules. We propose that colicins also differ with regard to mechanisms regulating gene expression providing a novel viewpoint of macromolecular evolution. In the framework of the proposed research project, comparison of the mechanisms regulating gene expression of several pore forming and endonucleolytic colicins will be performed. To elucidate the basis of plasmid carriage of colicin encoding genes the molecular structure of plasmid pColK-K235 will be studied. Colicins are interesting also with regard to its interaction with its cognate immunity protein. Studies of the interaction of colicin K with its immunity protein Cki will be inititated as a model of membrane protein-protein interactions.
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