Phospholipases in yeast Saccharomyces cerevisiae

Code

J1-6507 (C) - included in ARIS records

Head

PhD Igor Križaj

Period

7/1/2004 - 6/30/2007

Range in 2007

0.5 FTE

Science

Natural sciences and mathematics (5)

Reseacher status

Researcher (5)
Junior expert or technical associate (0)

Education

Doctor's degree (5)

Sex

Woman (1)
Man (4)

Status

Employed at RO and RRD (4)
Deceased (1)

No. of publications

100–999 (5)

Projects / Programmes source: ARIS

source: ARIS

Phospholipases in yeast Saccharomyces cerevisiae

Research activity

Code	Science	Field	Subfield
1.05.00	Natural sciences and mathematics	Biochemistry and molecular biology

Code	Science	Field
P004	Natural sciences and mathematics	Biochemistry, Metabolism

Keywords

yeast, Saccharomyces cerevisiae, phospholipases A2, transcriptome, signal transduction, cell cycle, obesity, cancer, neurotoxicity, DNA microarray

Evaluation (rules)

source: COBISS

Evaluation of bibliographic research performance indicators according to ARIS methodology

Citations Citations for bibliographic records in COBIB.SI that are linked to records in citation databases

source: WoS

source: Scopus

source: COBISS

Researchers (5)

no.	Code	Name and surname	Research area	Role	Period	No. of publicationsNo. of publications
1.	01083	PhD Franc Gubenšek	Biochemistry and molecular biology	Researcher	2004 - 2007	535
2.	00412	PhD Igor Križaj	Biochemistry and molecular biology	Head	2004 - 2007	725
3.	18802	PhD Adrijana Leonardi	Biochemistry and molecular biology	Researcher	2004 - 2007	156
4.	20653	PhD Uroš Petrovič	Biochemistry and molecular biology	Researcher	2004 - 2007	292
5.	21553	PhD Jernej Šribar	Biochemistry and molecular biology	Researcher	2004 - 2007	108

Organisations (1)

no.	Code	Research organisation	City	Registration number	No. of publicationsNo. of publications
1.	0106	Jožef Stefan Institute	Ljubljana	5051606000	90,664

Abstract

Understanding the regulation of lipid metabolism is of crucial importance in understanding cardiovascular diseases, atherosclerosis and lipid associated disorders, such as obesity. Involvement of phospholipase-mediated signal transduction pathways in these processes has been described in mammals, but the molecular basis behind that has not been completely elucidated. Known targets for secretory phospholipases A2 action are involved also in the regulation of the cell cycle and these interactions are therefore important in studying cancer. Yeast Saccharomyces cerevisiae as a model eukaryotic organism represents an important tool for studies on the regulation of the lipid metabolism on the molecular level, as well as that on other evolutionary conserved cellular processes, such as the regulation of the cell cycle. In the proposed project we will investigate phospholipase-mediated signalling in yeast, which has so far not been described in this species. Indications for such signal transduction pathway were found in our previous work when yeast was used as a model target cell for secretory phospholipases A2. Ammodytoxin, a neurotoxic phospholipase from the long-nosed viper venom will be used as a tool in our studies, but we will also focus on the yeast endogenous phospholipases. Besides, we will study the involvement of secretory phospholipases in the regulation of the cell cycle. This research will contribute to understanding the regulation of lipid metabolism and mechanisms of action of neurotoxic and non-toxic secretory phospholipases A2 in eukaryotic cells. At the same time, by using the cutting edge research techniques to study lipid metabolism on the whole-genome level, the project will contribute to development of research in the field of biochemistry and molecular biology in Slovenia and in Europe.