Projects / Programmes
Development and application of biosenzors in biological systems
| Code |
Science |
Field |
Subfield |
| 4.06.00 |
Biotechnical sciences |
Biotechnology |
|
| Code |
Science |
Field |
| T490 |
Technological sciences |
Biotechnology |
| P004 |
Natural sciences and mathematics |
Biochemistry, Metabolism |
| T360 |
Technological sciences |
Biochemical technology |
| B230 |
Biomedical sciences |
Microbiology, bacteriology, virology, mycology |
| P402 |
Natural sciences and mathematics |
Photochemistry |
Biosensors, molecular basis of the disease, physiology of microorganisms, metabolites, calcium dynamics, cell signalling, sepsis, immunity response
Researchers (6)
Organisations (1)
| no. |
Code |
Research organisation |
City |
Registration number |
No. of publicationsNo. of publications |
| 1. |
0104 |
National Institute of Chemistry |
Ljubljana |
5051592000 |
21,007 |
Abstract
Fluorescent probes are an excellent tool to monitor interactions among biomolecules and dynamics of signalling in biological systems with minimal perturbation in cell integrity and functionality under normal and pathological conditions. In vivo imaging techniques in combination with the immense information of genomics and proteomics will transform our understanding on cell biology. The exploitation of genetically encoded fluorescent probes for detection of calcium in filamentous fungi is in early stages. Therefore, we aim to develop genetically encoded fluorescent probes targeting physiological processes particularly focusing on cell signalling in biotechnologically important filamentous fungi. In parallel, fluorescent probes will be used also in mammalian cells focusing on endotoxin recognition in epithelial cells and monocytes/macrophages.
In our project we propose to develop and improve existing FRET -based calcium biosensors. In order to improve FRET, the donor- and acceptor- visible fluorescent proteins that are key components in these types of probes will be changed. The dynamics in intracellular concentration of free calcium ions will be measured in relation to mutations in calcium signalling pathway.
Another objective of the project is to use fluorescent probes for analysing and following interactions: between proteins (LPS induced aggregation of MD-2 and interaction MD-2/TLR4), and between proteins and signalling molecules (MD-2/LPS) and intercellular concentration of signalling molecules (free calcium ions, LPS). The fluorescent proteins (VFP), FlAsH and biosensors based on FRET will be used in studied systems such as mechanisms of endotoxin recognition through MD-2/TLR4, and than we want to answer the question on the development of di- or oligo-merisation of MD-2 in the process of endotoxin recognition,
We aim to develop high throughput screening methods based on calcium biosensors for testing anti-mycotics which effect calcium homeostasis and for testing compounds for prevention of sepsis by effecting oligo-merisation of MD-2/TLR4.
