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Projects / Programmes source: ARIS

Production of pharmaceutically active compounds from Grifola frondosa by solid state and submerged cultivation

Research activity

Code Science Field Subfield
4.06.00  Biotechnical sciences  Biotechnology   

Code Science Field
T490  Technological sciences  Biotechnology 
Keywords
biotehnology, Grifola frondosa, cultivation, solid substrate, liquid substrate, active compounds, isolation, testing, immumomodulatory effects
Evaluation (rules)
source: COBISS
Researchers (13)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  06110  PhD Marin Berovič  Biotechnology  Head  2004 - 2007  748 
2.  04566  PhD Bojana Boh Podgornik  Chemistry  Researcher  2004 - 2007  832 
3.  21332  PhD Žiga Bolta  Biotechnology  Researcher  2004  24 
4.  12090  PhD Danica Dolničar  Computer science and informatics  Researcher  2004  178 
5.  20448  PhD Vesna Ferk Savec  Educational studies  Researcher  2004 - 2006  419 
6.  28538  Katarina Jordan    Technical associate  2007 
7.  21917  Dušan Komel    Technical associate  2005 - 2007 
8.  12681  PhD Bojan Pahor  Control and care of the environment  Researcher  2005 - 2007  166 
9.  20043  PhD Aleksandra Pivec  Chemical engineering  Researcher  2005 - 2007  107 
10.  05248  PhD Franc Pohleven  Forestry, wood and paper technology  Researcher  2004 - 2007  1,195 
11.  15328  PhD Kristina Sepčić  Biochemistry and molecular biology  Researcher  2004 - 2007  729 
12.  04890  PhD Branka Wraber  Microbiology and immunology  Researcher  2004 - 2007  266 
13.  20457  Andreja Žagar    Technical associate  2004 - 2007  62 
Organisations (5)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0103  University of Ljubljana, Faculty of Chemistry and Chemical Technology  Ljubljana  1626990  23,064 
2.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  48,188 
3.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  66,240 
4.  1421  Scientific research Centre Bistra Ptuj  Ptuj  5844975000  431 
5.  1555  University of Ljubljana, Faculty of Natural Sciences and Engeneering  Ljubljana  1627074  19,817 
Abstract
Grifola frondosa is a lignin degrading Basidiomycete, an edible and pharmaceutically promising mushroom. In the proposed project, strains of G. frondosa from different geographic regions in Slovenia and China will be taxonomically characterised, and best productive basic cultures will be selected for cultivation. Three different biotechnological processes will be applied and optimised: (1) submerged cultivation in a stirred tank bioreactor for production of active fungal biomass in a liquid phase, (2) controlled solid state cultivation of fungal biomass in a horizontal bioreactor, (3) fruiting bodies production in polyethylene bags filled with sterile secondary raw materials from wood industry and agriculture (straw, woodcuts, wheat bran). Extraction and isolation of the main groups of pharmacologically active substances (extra- and intracellular polysaccharides, proteoglycans) from fruiting bodies and mycelium biomass will be adapted and optimised. Immunomodulatory effects (induction of IL, IF and TNF) of purified extracts and/or isolated active compounds will be tested in vitro on human cell lines (PBMC). Project results will contribute to the scientific knowledge about immunomodulatory compounds from Griffola, and to the potential development of immunostimulatory pharmaceuticals for human and veterinary use.
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