Projects / Programmes
Peptide ligands as novel biopharmaceutics by using phage display technology
| Code |
Science |
Field |
Subfield |
| 4.06.00 |
Biotechnical sciences |
Biotechnology |
|
| Code |
Science |
Field |
| T490 |
Technological sciences |
Biotechnology |
| B740 |
Biomedical sciences |
Pharmacological sciences, pharmacognosy, pharmacy, toxicology |
phage disoplay library, ligand, enzyme, inhibitor
Researchers (2)
| no. |
Code |
Name and surname |
Research area |
Role |
Period |
No. of publicationsNo. of publications |
| 1. |
19785 |
PhD Bojan Doljak |
Pharmacy |
Researcher |
2004 - 2006 |
257 |
| 2. |
07849 |
PhD Borut Štrukelj |
Biochemistry and molecular biology |
Head |
2004 - 2006 |
1,125 |
Organisations (1)
Abstract
Phage display libraries are collections (mixtures) of large number of bacteriophage clones acquired by molecular biological techniques. The bacteriophages express an peptide exposed on coat protein. Every clone expresses one type of peptide with exactly defined amino acid sequence. Due to the exposed peptide the phages which belong to different clones, bind on target molecule with differently strength. Their affinity to target molecule depends on affinity of peptide they express.The property is used for selection of clones, with high affinity. Phage display libraries are therefore effective for selection of peptide ligands, which form strong interactions with target molecules. Strong interaction are formed by e.g. antibodies and proteins from group of avidins. In the framework of this project will explore of possibility for use phage display libraries for selection of ligands, which form weaker interaction with target molecules. Such interactions are expected in the enzyme-inhibitor complex. A possibility to use capillary electrophoresis in selection of phage clones will be explored.
