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Projects / Programmes source: ARIS

Contemporary diagnosis of american foulbrood, pestis apium

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Research activity

Code Science Field Subfield
4.04.02  Biotechnical sciences  Veterinarian medicine  Animal pathology and epizootiology 

Code Science Field
B750  Biomedical sciences  Veterinary medicine: surgery, physiology, pathology, clinical studies 
Keywords
Honeybee, Apis mellifera, American foulbrood, Paenibacillus larvae, immunohistology, cytology
Evaluation (rules)
source: COBISS
Evaluation of bibliographic research performance indicators according to ARIS methodology
Citations Citations for bibliographic records in COBIB.SI that are linked to records in citation databases
source: WoS source: Scopus source: COBISS
Researchers (1)
no. Code Name and surname Research area Role Period No. of publications
1.  10448  PhD Aleš Gregorc  Animal production  Head  1998 - 2001  500 
Organisations (1)
no. Code Research organisation City Registration number No. of publications
1.  0406  University of Ljubljana, Veterinary Faculty  Ljubljana  1627139  10,756 
Abstract
Our previous studies of honeybee biology and pathology have raised several issues that would be of relevance for future studies. We studied programmed cell death in honeybee, Apis mellifera, larvae midgut and histological and morphological changes in the larvae after Paenibacillus larvae infection. This is a causative agent of American foulbrood disease. Immunohistochemical and cytochemical methods are used to study physiological and pathological - necrotic cell death mechanisms. We developed polyclonal antibodies against Paenibacillus larvae and we used ELISA method to demonstrate antibody specificity. Free acid phosphatase has been detected, histone group protein and Heat shock protein (Hsp70 Hsp90) were localised in hineybee larvae midgut cells. We have tested different immunohistochemical diagnostic methods to distinguish between programmed and pathological, necrotic cell death. In our future research our interest is focused on diagnosis and detection of the effects of external organic and inorganic influences on honeybee larvae development and the development of the entire honeybee colony. The main aim of our research is to determine the cellular, subcellular and histopathological effects of commonly used pesticides on honeybee larval tissues. Our interest is focused on analyzing the physiological and pathological process at morphological and immunohistochemical level and to evolve suitable diagnostic techniques.
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