Projects / Programmes
Development of protein microarray for research on gastric cancer proteome
| Code |
Science |
Field |
Subfield |
| 1.05.00 |
Natural sciences and mathematics |
Biochemistry and molecular biology |
|
| Code |
Science |
Field |
| P004 |
Natural sciences and mathematics |
Biochemistry, Metabolism |
| B200 |
Biomedical sciences |
Cytology, oncology, cancerology |
gastric cancer, oncogenesis, protein microarrays, bio-chips, protein markers, oncoproteins, proteome
Researchers (8)
| no. |
Code |
Name and surname |
Research area |
Role |
Period |
No. of publicationsNo. of publications |
| 1. |
18622 |
PhD Nataša Debeljak |
Biochemistry and molecular biology |
Researcher |
2007 - 2009 |
241 |
| 2. |
18529 |
Dubravka Germ |
|
Technical associate |
2007 - 2009 |
0 |
| 3. |
21395 |
PhD Petra Hudler |
Medical sciences |
Researcher |
2007 - 2009 |
152 |
| 4. |
13839 |
MSc Robert Juvan |
Oncology |
Researcher |
2007 - 2009 |
188 |
| 5. |
28916 |
PhD Damjana Kastelic |
Biochemistry and molecular biology |
Researcher |
2008 - 2009 |
33 |
| 6. |
24562 |
Helena Klavžar |
Biochemistry and molecular biology |
Technical associate |
2007 - 2009 |
0 |
| 7. |
06135 |
PhD Radovan Komel |
Biochemistry and molecular biology |
Head |
2007 - 2009 |
1,054 |
| 8. |
01528 |
PhD Stanislav Repše |
Oncology |
Researcher |
2007 - 2009 |
308 |
Organisations (2)
Abstract
The incidence and mortality of gastric cancer have fallen in most of the developed countries, but nonetheless, it remains a major public health issue as the fourth most common cancer and the second leading cause of cancer death worldwide. Due to the oligosymptomatic course of early gastric disease, most cases are diagnosed in the advanced stages.
Our intention is to combine the transcriptome level with the protein expression patterns in gastric tumor cells in order to aid in determination of molecular principles of the disease development. The purpose of this study is to develop a protein onco-chip as a new diagnostic tool for early detection of phenotypic markers of gastric cancer cells and precancerous lesions on the proteomic level based on a on a new technology: IRPS (analysis with surface plasmon resonance). This approach enables analysis of proteins ‘in real time’ with no need for special labelling, and also investigation of protein-protein interactions. In parallel, for finding correlations between gene expression and cellular protein content, as well as to assess experiments with the protein onco-chip, transcriptome of gastric cancer will be studied with low-density DNA microarray constructed on the basis of specific group of markers selected from previous studies.
We hope to identify important common biomarkers that could enable better understanding of molecular mechanisms of cancerogenesis as well as be potentially important for cancer diagnosis.
Significance for science
The aim of the project was selection of new protein markers for the different development stages of stomach adenocarcinome and its precancerous lesions, and use of these markers in conjunction with classical markers for the design of a protein onco-chip usable as a diagnostic tool for early detection of differential expression patterns of proteins constituting specific signatures in cancer. Results will provide a better basic insight into molecular mechanisms of cancerogenesis and will allow to define a new set of markers corresponding to normal and pathologic physiological states. Developed protein chip will represent valuable prototype for further development of investigative or commercial diagnostic microarrays.
When analysing llama VHH protein sequences we develped a computer algorithm based on the discovery of co-evolution of amino acid residues located on the antibody surface, with certain a.a. residues directly implicated in antigen recognition. This discovery could have a high value in recombinant antibody technology, since we can predict and design antigen recognition site from amino acid sequence of an antibody.
Highly specific antibodies and/or their hypervariable single-chain doomains belong to top priority research in molecular oncology, because of the expected usefulness in immunotherapy and cancer treatment as well as in design and development of new accurate diagnostic procedures.
Significance for the country
Proteomic approach for looking for specific markers of oncogenesis is introducing new methodology of the post-genomic science to Slovenian medical research and practice.
Better insight into molecular mechanisms of oncogenesis and improved selection of markers corresponding to normal and pathologic physiological states will offer possibilities for more efficient early detection of cancer, prediction and follow-up of the disease, and also better therapeutic approach. The interdisciplinary development of a protein microarray is associating approaches of functional genomics, medical molecular biology, nanotechnology and bioinformatics. The developed bio-chips will allow further improvement of research in molecular oncology and will represent valuable prototypes for commercial diagnostic microarrays. The project is also creating starting positions for creation of spin-offs for the development of new diagnostic and therapeutic tools.
Most important scientific results
Annual report
2008,
final report,
complete report on dLib.si
Most important socioeconomically and culturally relevant results
Annual report
2008,
final report,
complete report on dLib.si
