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Projects / Programmes source: ARIS

Fertility after testicular cancer treatment

Research activity

Code Science Field Subfield
3.05.00  Medical sciences  Human reproduction   

Code Science Field
B570  Biomedical sciences  Obstetrics, gynaecology, andrology, reproduction, sexuality 
B520  Biomedical sciences  General pathology, pathological anatomy 
Keywords
Testicular biopsy/DNA/ejaculate/gametes/histology/hormones/testis/fertility/cancer/in vitro fertilization
Evaluation (rules)
source: COBISS
Researchers (20)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  10568  Sašo Drobnič  Human reproduction  Researcher  2007 - 2009  137 
2.  07652  PhD Vislava Globevnik Velikonja  Human reproduction  Researcher  2007 - 2009  316 
3.  17635  Klementa Habjan    Technical associate  2007 - 2009 
4.  28277  Milena Janezič    Technical associate  2007 - 2009 
5.  03721  Mojca Kolbezen Simoniti  Public health (occupational safety)  Researcher  2007 - 2009  43 
6.  20055  PhD Erika Matos  Oncology  Researcher  2007 - 2009  186 
7.  28278  Alenka Mitrovič    Technical associate  2007 - 2009 
8.  13089  PhD Ciril Oblak  Human reproduction  Researcher  2007 - 2009  133 
9.  10691  PhD Joško Osredkar  Human reproduction  Researcher  2007 - 2009  1,305 
10.  22159  Martina Pečlin    Technical associate  2007 - 2009 
11.  05984  PhD Marija Pfeifer  Metabolic and hormonal disorders  Researcher  2007 - 2009  381 
12.  14534  Mojca Pirc  Human reproduction  Researcher  2007 - 2009  23 
13.  17648  Draga Plavc    Technical associate  2007 - 2009 
14.  07183  Jasna Šinkovec  Human reproduction  Researcher  2007 - 2009  134 
15.  20177  MSc Andreja Cirila Škufca Smrdel  Oncology  Researcher  2007 - 2009  168 
16.  28279  Katarina Turkalj    Technical associate  2007 - 2009 
17.  12245  Alenka Veble  Human reproduction  Researcher  2007 - 2009  70 
18.  05015  PhD Ivan Verdenik  Human reproduction  Researcher  2007 - 2009  479 
19.  20257  PhD Karin Writzl  Human reproduction  Researcher  2007 - 2009  207 
20.  19449  PhD Branko Zorn  Human reproduction  Head  2007 - 2009  307 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0302  Institute of Oncology Ljubljana  Ljubljana  5055733000  15,390 
2.  0312  University Medical Centre Ljubljana  Ljubljana  5057272000  76,701 
Abstract
After treatment of testicular cancer, 30 % of these young men become infertile. Only 5 to 10 % of them use sperm cryopreserved before cytotoxic treatment. Beside oncological treatment, other factors, i.e. primary testicular disease or histologic type of tumour are supposed to affect spermatogenesis. Impairment of sperm DNA integrity and mutagenic effect on chromosomes might be the causes of infertility. The aims of the study are to research i) how patients manage the infertility problem, ii) what are the clinical and histologic prognostic factors of spermatogenesis impairment, and iii) what are the extent and duration of sperm DNA and chromosome damage. In this 3-year prospective study, 50 men after orchidectomy will be enrolled yearly, and controlled at orchidectomy, at sperm cryopreservation, at 3, 6, 12 and 24 months after orchidectomy with histologic scoring of spermatogenesis, clinical examination and psychologic testing, hormonal evaluation, sperm DNA fragmentation and testing for aneuploidy of chromosomes X, Y, 13, 18 and 21 using FISH method. Psychologic testing will clarify the mechanisms of fight against infertility. Histologic scoring of spermatogenesis will be performed at the same time as the histologic evaluation of the cancer lesion and compared with the effects related to treatment; it may be used as a predictive factor of fertility potential. The amplitude and duration of sperm DNA fragmentation and chromosomal aneuploidy will permit us to counsel patients adequately in terms of fertility and offspring risks.
Significance for science
The preliminary analysis indicates the important changes in sex hormone levels, especially in FSH in patients treated for testicular cancer. The final analysis will be focussed on the effect of testosterone deficiency and oscillation of FSH on sperm quality and on patients' psychologic characteristics.
Significance for the country
This research has already provided new knowledge in pathogenesis of changes, triggered by testicular cancer, affecting general physical, mental, sex and reproductive status of young men. Thus, the final analysis will help therapists to be better equipped for helping the patients in improving their general well-being.
Most important scientific results Annual report 2008, final report, complete report on dLib.si
Most important socioeconomically and culturally relevant results Annual report 2008, final report, complete report on dLib.si
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