Projects / Programmes source: ARIS

The biotechnological control of the tomato fruit abscission for a higher crop yield

Research activity

Code Science Field Subfield
4.06.00  Biotechnical sciences  Biotechnology   

Code Science Field
T490  Technological sciences  Biotechnology 
fruit abscission; tomato; progammed cell death; RNase LX; biotechnological control
Evaluation (rules)
source: COBISS
Researchers (7)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  08280  PhD Marina Dermastia  Biology  Head  2007 - 2009  872 
2.  27827  PhD Jon Gutierrez Aguirre  Biotechnology  Researcher  2007 - 2009  363 
3.  20070  PhD Aleš Kladnik  Biology  Researcher  2007 - 2009  174 
4.  04333  PhD Maja Kovač  Biotechnology  Researcher  2009  204 
5.  18467  PhD Maruša Pompe Novak  Biotechnology  Researcher  2007 - 2009  291 
6.  05229  PhD Maja Ravnikar  Biotechnology  Researcher  2007 - 2009  1,369 
7.  29635  Katja Stare  Biology  Technical associate  2009  86 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0105  National Institute of Biology  Ljubljana  5055784  13,239 
2.  0481  University of Ljubljana, Biotechnical Faculty  Ljubljana  1626914  66,240 
Abscission has a large agricultural implication during plant growth, as well as during storage and shelf life of the fresh produce. In different agricultural commodities there is a need to either inhibit or induce abscission. Premature abscission of flowers or young fruits in trees has a negative implication for yield while acceleration of abscission in different fresh produce during postharvest life and storage results in significant loss of quality. Acceleration of abscission may be required at a given time to allow improved mechanized fruit harvesting or leaf removal. Dehiscence mediating dispersal of mature seeds from the silique is believed to share common biochemical and molecular features with abscission. Recently we have demonstrated that the tomato LX RNase is involved in and required for abscission to occur and suggest that programmed cell death (PCD) is involved in abscission. Based on these novel findings we propose to develop a new biotechnological approach to manipulate the abscission process in tomato by modulating LX expression in the established tomato lines. The occurrence of PCD in the abscission zone and the specific involvement of LX will be investigated.
Significance for science
The new findings regarding the abscission and dehiscence processes in plants derive mainly from studying Arabidopsis petal abscission and it is not clear how knowledge from this system will be relevant to fruit and leaf abscission in crop plants such as tomato. The applied tools and the results of RNase LX involvement in abscission together with the development of PCD in the abscission layer may be applied to various relevant crops. The problem of organ separation and dehiscence has large economical implications in different crop plants and the ability to manipulate these processes using biotechnology can has large economical implication. It is hard at this early stage to estimate the size of economic contribution but even if it could be applied in tomato it will have significant economical contribution.
Significance for the country
The proposed research might lead toward advance use of biotechnology in crops plants for the benefit of agriculture and the relevant economy.
Most important scientific results Annual report 2008, final report, complete report on dLib.si
Most important socioeconomically and culturally relevant results Annual report 2008, final report, complete report on dLib.si
Views history