Projects / Programmes
The effect of normobaric and hyperbaric oxygen on brain neuronal and astrocytic cell apoptosis and serum levels of S100B protein in carbon monoxide poisoning.
| Code |
Science |
Field |
Subfield |
| 3.03.00 |
Medical sciences |
Neurobiology |
|
| Code |
Science |
Field |
| B740 |
Biomedical sciences |
Pharmacological sciences, pharmacognosy, pharmacy, toxicology |
carbon monoxide, hyperbaric oxygen therapy, brain injury, neuronal necosis, neuronal apoptosis, astrocytic cell apoptosis, biochemical marker, s100b protein
Researchers (6)
| no. |
Code |
Name and surname |
Research area |
Role |
Period |
No. of publicationsNo. of publications |
| 1. |
24927 |
PhD Miran Brvar |
Neurobiology |
Head |
2008 - 2011 |
599 |
| 2. |
15667 |
PhD Matjaž Bunc |
Neurobiology |
Researcher |
2008 - 2011 |
530 |
| 3. |
10779 |
PhD Rok Černe |
Neurobiology |
Researcher |
2008 - 2011 |
39 |
| 4. |
15169 |
PhD Damijana Mojca Jurič |
Neurobiology |
Researcher |
2008 - 2011 |
103 |
| 5. |
01984 |
Martin Možina |
Neurobiology |
Researcher |
2008 - 2011 |
228 |
| 6. |
07002 |
PhD Dušan Šuput |
Neurobiology |
Researcher |
2008 - 2011 |
433 |
Organisations (2)
Abstract
Carbon monoxide (CO) is the leading cause of poisoning-related death and morbidity. CO poisoning results in brains neuronal and astrocytic necrosis and apoptosis. Treatment includes normobaric and hyperbaric oxygen. However the usefulness of normobaric and hyperbaric oxygen in CO-poisoned patients has not been proven yet. Interestingly, there is no published research about in vitro normobaric and hyperbaric oxygen efficiency in preventing neuronal and astrocytic cells necrosis and apoptosis due to CO exposure. Nevertheless in present clinical practice normobaric or/and hyperbaric oxygen therapy is commonly used in CO-poisoned patients, but indications for hyperbaric oxygen therapy of CO poisoned patients are mainly clinical and subjective. Therefore it would be convenient to have an objective biochemical marker that would help us in the decision concerning hyperbaric-oxygen therapy. The aim of our proposed project is to evaluate normobaric and hyperbaric oxygen efficiency in preventing neuronal and astrocytic cell apoptosis after CO exposure and to assess the possible role of S100B as a biochemical marker and prognostic parameter in CO poisoning. In the first part of our project we are planning to evaluate in vitro efficacy of normobaric and hyperbaric oxygen therapy in preventing neuronal and astrocytic cell apoptosis in different time periods after CO exposure. In the second part of our project we will evaluate serum S100B levels and histological brain changes in an animal model of CO-poisoning and S100B levels, clinical picture, neuropsychological testing and brain magnetic resonance imaging in CO-poisoned patients.The finding of hyperbaric oxygen time dependent protective effects on CO-induced neuronal and astrocytic cell apoptosis would be breakthrough in the research of hyperbaric oxygen usefulness in CO poisoning. This could lead to the modification of current indications for hyperbaric oxygen and rationalization of hyperbaric oxygen use. The results about S100B protein in CO-poisoning might present a new promising direction in the search for biochemical markers and prognostic parameters that would help us in the evaluation of severity and therapy decisions in CO poisoning.
Significance for science
The finding of the optimum timing of oxygen therapy in CO-induced neuronal and astrocytic cell apoptosis is a breakthrough in the research of oxygen therapy in CO poisoning. These could lead to the modification of currently used indications for hyperbaric oxygen in CO-poisoned patients according to the time elapsed after CO exposure.
Additional findings about the optimum percentage of oxygen therapy on neuronal and astrocytic cell apoptosis would lead to the modification of currently used normobaric and hyperbaric oxygen protocols in CO-poisoned patients. According to our studies we expect therapy with a low oxygen dose immediately after CO exposure followed by a prolonged high oxygen dose to give superior results in the prevention of CO-induced brain injury.
In CO poisoning S100B protein is useful as biochemical marker and objective criteria for hyperbaric oxygen therapy. S100B protein is already used in treatment of CO poisoned patients in UMC Ljubljana. The decision concerning hyperbaric oxygen therapy in the Emergency Department is much easier and more objective by measuring serum S100B levels.
Significance for the country
The indications for hyperbaric oxygen therapy of CO-poisoned patients adjusted according to the time elapsed after CO exposure and objective measurements of serum S100B protein will reduce the frequency of hyperbaric oxygen therapy and, consequently, reduce its cost. The decisions concerning hyperbaric oxygen therapy have become much easier and more objective.
Additional improvement of normobaric and hyperbaric oxygen protocols in CO poisoning regarding the finding of the optimum timing will further reduce the frequency of late neuropsychological sequelae after CO poisoning and reduce the cost of subsequent treatment and rehabilitation of CO-poisoned patients in Slovenia and the European Union where CO poisoning is still the leading cause of death due to poisoning.
The research project has promoted Slovenian basic and clinical toxicology in the scientific and health care communities.
Better knowledge regarding the danger of CO, such as cognitive sequelae, will lead to the introduction of CO detectors and alarms and spread of improved educational and preventive programs and campaigns throughout Europe.
Most important scientific results
Annual report
2008,
2009,
final report,
complete report on dLib.si
Most important socioeconomically and culturally relevant results
Annual report
2008,
2009,
final report,
complete report on dLib.si
