Projects / Programmes source: ARIS

The Influence of Ultrasound Exposure on Development of Rat Fetuses

Research activity

Code Science Field Subfield
3.03.00  Medical sciences  Neurobiology   

Code Science Field
B450  Biomedical sciences  Development biology, teratology, ontogeny, embryology (human) 
Ultrasound, rat fetuses, nerve tissues, apoptosis, PCR arrays, injuries of nerve tissues, heart injuries, pharmacological modulation of injuries
Evaluation (rules)
source: COBISS
Researchers (8)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  13310  PhD Gorazd Drevenšek  Neurobiology  Researcher  2008 - 2009  328 
2.  18122  Boštjan Drolc  Veterinarian medicine  Technical associate  2008 - 2011  10 
3.  12449  PhD Robert Frangež  Veterinarian medicine  Researcher  2008 - 2011  274 
4.  29315  PhD Zvonko Hočevar  Public health (occupational safety)  Researcher  2008 - 2011  49 
5.  21395  PhD Petra Hudler  Medical sciences  Researcher  2010 - 2011  144 
6.  21175  PhD Jernej Kužner  Veterinarian medicine  Researcher  2008 - 2009  96 
7.  04293  PhD Janez Rozman  Systems and cybernetics  Head  2008 - 2011  245 
8.  03250  PhD Vladimir Smrkolj  Neurobiology  Researcher  2008 - 2011  196 
Organisations (4)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0312  University Medical Centre Ljubljana  Ljubljana  5057272000  75,496 
2.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  45,382 
3.  0406  University of Ljubljana, Veterinary Faculty  Ljubljana  1627139  10,132 
4.  1326  ITIS, Implantable Technology and Sensors, producing, trading, servicing and researching enterprise, d.o.o. Ljubljana  Ljubljana  5824796000  245 
Embryos in the early stages of development are very sensitive for physical stress. When non-thermal effects of ultrasound undulation are excluded, the use of ultrasound examination must be set very cautiously during the first three months of pregnancy, especially if the extra uterine fertilization was done and embryos implanted. The influence of overexposure to ultrasound examination to psychomotor, behavioral and developmental capacities of organism are most easily studied on the developmental stage of the fetuses, when they are in the most fastest development of target tissues and organs. During the injuries of central nervous system and cardiovascular system unwanted influences are most easily studied in the embryonic stage of development, since the central nervous and cardiovascular system are most vulnerable to ultrasound exposure. Ultrasound will be studied on development of central nervous system and heart during early stages of embryonic development. There are practically no studies where ultrasound is evaluated for influences to embryos. Most sensitive markers of biological influences are developmental markers in embryos. The model of pregnant rats exposed to ultrasound will be used. Cellular and tissue effects of ultrasound will be focused in our study by use of molecular techniques in early developmental stages of embryos, by evaluation of markers, responsible for nervous development and demonstrating changes and injuries in these tissues. Genes and segments of DNA where overexpression will be determined, showing potential harmful effects of ultrasound. Markers of apoptosis will be used to evaluate the influence of ultrasound on cells.
Significance for science
Potential biological effects of ultrasound waves on developmental, psychomotoric as well as behavioural capabilities of individuals, exposed to ultrasound waves, are still not adequately described. In procedures of fertilization with biomedical assistance and in control with ultrasound waves it is crucial to know all potential effects onto key phases in development of a central neural system. The developed and tested animal model gives some answers on possible effects of ultrasound waves onto central nervous system of rat embryos. Testing of the hypothesis provided sufficient qualitative and quantitative data on various gene expression. Therefore, it could be expected that at the end of the project, results will represent some contribution to formulation of recommendations to be used in procedures of fertilization with biomedical assistance and in control with ultrasound waves.
Significance for the country
By confirming the hypothesis, in which ultrasound waves affect a development of central naural system of rat embryos in early steges, some positive effects on technological and social development of the Republic of Slovenia were obtained. In a technological sense, the results enhanced the knowledge of ultrasound users and developers of new more sophisticated ultrasound machines and methods in the Republic of Slovenia. In public sense, obtained results could significantly contribute to formulation of recommendations to be used in procedures of fertilization with biomedical assistance and in control with ultrasound waves in the Republic of Slovenia and elsewhere.
Most important scientific results Annual report 2008, 2009, final report, complete report on dLib.si
Most important socioeconomically and culturally relevant results Annual report 2008, 2009, final report, complete report on dLib.si
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