Projects / Programmes source: ARIS

Development of treatments and procedures for improvement of hemocompatibility of polyethyleneterephthate surfaces

Research activity

Code Science Field Subfield
2.14.02  Engineering sciences and technologies  Textile and leather  Textile chemistry 

Code Science Field
T470  Technological sciences  Textiles technology 
T390  Technological sciences  Polymer technology, biopolymers 
polietilentereftalat, bioaktivnost, hemokompatibilnost, aktivacija s plazmo, biopolimeri
Evaluation (rules)
source: COBISS
Researchers (26)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  29765  PhD Miha Antonič  Medical sciences  Researcher  2008 - 2010  91 
2.  01694  Zlatko Bele  Engineering sciences and technologies  Researcher  2008 - 2011  17 
3.  19753  PhD Lidija Fras Zemljič  Engineering sciences and technologies  Researcher  2008 - 2011  508 
4.  27558  PhD Silvo Hribernik  Engineering sciences and technologies  Researcher  2008 - 2011  293 
5.  24334  PhD Tea Indest  Engineering sciences and technologies  Researcher  2008 - 2011  35 
6.  22403  PhD Marko Jevšek  Medical sciences  Researcher  2008 - 2010  117 
7.  28480  PhD Ita Junkar  Medical sciences  Researcher  2008 - 2011  277 
8.  29767  Peter Jurič  Medical sciences  Researcher  2008 - 2010  16 
9.  28976  Tanja Kos    Technical associate  2008 - 2011  42 
10.  15724  Gorazd Košir  Medical sciences  Researcher  2008 - 2011  61 
11.  15703  PhD Janez Kovač  Engineering sciences and technologies  Researcher  2008 - 2011  656 
12.  04629  PhD Tatjana Kreže  Engineering sciences and technologies  Researcher  2008 - 2011  276 
13.  29766  PhD Juš Kšela  Medical sciences  Researcher  2008 - 2010  165 
14.  24332  PhD Manja Kurečič  Engineering sciences and technologies  Junior researcher  2008 - 2011  217 
15.  16402  PhD Saška Lipovšek  Medical sciences  Researcher  2008 - 2011  185 
16.  17579  Mitja Mlakar    Technical associate  2008 - 2011  43 
17.  23660  Rudi Mlakar  Engineering sciences and technologies  Technical associate  2008 - 2011 
18.  10429  PhD Miran Mozetič  Engineering sciences and technologies  Researcher  2008 - 2011  1,332 
19.  19268  PhD Zdenka Peršin Fratnik  Engineering sciences and technologies  Researcher  2008 - 2011  225 
20.  09105  Borut Praček  Engineering sciences and technologies  Researcher  2008 - 2011  113 
21.  04171  PhD Majda Sfiligoj Smole  Engineering sciences and technologies  Researcher  2008 - 2011  433 
22.  12266  PhD Marjan Slak Rupnik  Medical sciences  Researcher  2008 - 2010  346 
23.  07814  PhD Karin Stana Kleinschek  Engineering sciences and technologies  Head  2008 - 2011  1,110 
24.  10575  PhD Simona Strnad  Engineering sciences and technologies  Researcher  2008 - 2011  367 
25.  07003  PhD Olivera Šauperl  Engineering sciences and technologies  Researcher  2008 - 2011  407 
26.  20048  PhD Alenka Vesel  Engineering sciences and technologies  Researcher  2008 - 2011  675 
Organisations (6)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0106  Jožef Stefan Institute  Ljubljana  5051606000  87,078 
2.  0334  University Medical Centre Maribor  Maribor  5054150000  22,681 
3.  0341  MIKROIKS, microelectronics engineering, consulting and service  Ljubljana  5286646  70 
4.  0795  University ob Maribor, Faculty of mechanical engineering  Maribor  5089638010  24,299 
5.  2334  University of Maribor, Faculty of Medicine  Maribor  5089638048  16,091 
6.  2547  University of Maribor, Faculty of natural sciences and mathematics  Maribor  5089638051  17,650 
The surface structure of biomaterial governs the biological response, which is initially determined by protein and cell interactions. It is though understandable that the aim is to modify surface chemistry of polymers in contact with blood, to optimize the cell-polymer interactions. The commonality among biomaterials, used for medical application (i.e. gold, polyurethane, silicone, rubber, polytetrafluoroethylene,…) is that they adsorb a complex, non-specific layer of proteins, which in nature does not occure. Many strategies to inhibit such a non-specific protein adsorption have been developed and used, like pre-treatment with proteins, use of hydrogels and saccharides, improvement of physico chemical properties, incorporation of negative charges, application of anticoagulant and antiplatelet agents, lining of materials surface with endothelial cells. To the best of our knowledge, no biomaterial has yet shown satisfactory performances when in contact with blood for long time periods and so far no appropriate biomaterial has been developed for small diameter vessel replacements. Thus, the common clinical complications that occur after implantation, such as aneurysms, thrombosis or restenosis are very often. The aim of this project is the development of procedures for modifications of PET surface with the introduction of new natural antithrombotic substances in order to improve its hemocompatibility. The strategy to overcome known problems is to modify grafts surface charge and surface energy in order to mimic surface properties similar to natural vessels. This shall be obtained using physical methods of surface modification followed by surface coating with chosen natural polysaccharides with promising antithrombotic properties (derivatized chitosan, fucoidan). In the frame of the project meaningful surface characterisation will be performed and in this way better knowledge about the influences of different treatment conditions on the adsorption of various combinations of biopolimers on the PET surface will be achieved and in such a way some obscurities about the interdependence between biomaterials surface properties and its biocompatibility will be clarified.
Significance for science
The usability of the alternative (natural) antithrombogen substances for the synthetic materials surfaces modification, i.e. poly(ethylene terephthalate; PET) was studied. PET is frequently used material for production of synthetic vascular grafts as well as for medical devices, which are used for the external blood circulation. The most important knowledge for the scientific development achieved in this project is the knowledge about the application and chemical modification of different alternative polysaccharides for production of antithrombotic surfaces. The use of chitosan, fucoidan, dextran and galactoglucomannan for the development of antithrombogen surfaces represents a new approach. Beside above mentioned substances sulphated chitosan, sulphated galactoglucomannan and cellulose were studied. It was established that the amount of sulphated groups is proportional to the antithrobmbogenic effects. Chemically modified (sulphated) PS were modified in the way, that their antithrombogenic properties reach that ones of heparin. These scientific achievements contribute to the progress in searching for new solutions in biomaterials’ hemocompatibility improvement. QCM technique was studied in order to its application for in-vitro determination of hemocompatibility with the protein adsorption onto synthetic polymers’ surfaces. The “released haemoglobin method” was optimised regarding pH of incubation solution, haemoglobin dispersion in solution, anticoagulant addition and blood cooling. This optimization procedure enabled application of the method for in-vitro testing of hemocompatibility of chemically similar surfaces. The main problem in the field of biomaterials hemocompatibility is still the in-vitro evaluation of the real surfaces. The project results essentially contributed to the development of these methods. The method of of hemocompatibility determination of model surfaces with the analysis of protein adsorption and investigation of the coagulation process of adsorbed layer using quartz crystal microbalance with dissipation unit was successfully introduced. Furthermore, the “haemoglobin free method”, which is suitable for real surfaces was introduced and optimised. Beside this the study of the applicability of the determination of the form and number of adhered thrombocytes onto real polymer surface was performed. The results achieved in this project essentially contributed to better understanding of the processes, which are triggered after the contact of real biomaterials’ surfaces with blood.
Significance for the country
Ability to use polysaccharides for achievement of antithrombogen characteristics of PET surfaces shows potential development of new technology. Development of procedures for preparation of alternative polysaccharides for hemocompatible (antithrombogenic) PET surfaces preparation is among the priorities defined in Slovenian national research program. The application of the polysaccharides and techniques prepared and modified in this project, will significantly contribute to the decrease of post-surgical complications and will consequently lower the time spent in the hospital and markedly improve overall quality of a patient’s life. Rehabilitation of patients will be better and quicker and they will be returned to their private care. This will result in the disburdening of the health care system. The results of the project represent large information database about the antithrobotic effects of new alternative sulphated polysaccharides. This will be the basis for the development of new sustainable biomaterials and establishment of SMEs for their production in Slovenia. The development of the methods for the determination of the antithrombotic properties of real surfaces represent the precious platform for further research of materials and substances, which could in the future replace conventional antithrombotic drugs based on animal sources and is a novelty in Slovenia area.
Most important scientific results Annual report 2008, 2009, final report, complete report on dLib.si
Most important socioeconomically and culturally relevant results Annual report 2008, 2009, final report, complete report on dLib.si
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