Projects / Programmes
DISTRIBUTION OF HUMAN PAPILLOMA VIRUS TYPES AND SUBTYPES IN PATIENTS WITH CERVICAL CANCER
| Code |
Science |
Field |
Subfield |
| 3.04.00 |
Medical sciences |
Oncology |
|
| Code |
Science |
Field |
| B200 |
Biomedical sciences |
Cytology, oncology, cancerology |
| B570 |
Biomedical sciences |
Obstetrics, gynaecology, andrology, reproduction, sexuality |
cancer of the uterine cervix, human papiloma virus (HPV), precancerous cervical lesions, subtypes of HPV genotype
Researchers (22)
Organisations (1)
Abstract
Background: Cancer of the uterine cervix is the second most frequent cancer in women. It is known that the development of cervical cancer requires a longterm infection with high-risk human papilloma virus (HPV) genotypes. Currently, almost 100 HPV genotypes are known, but only about 20 are involved in the development of cervical cancer. Since a longterm infection and cervical cancer develop only in a small share of women infected with these genotypes, it is very likely that within a certain genotype there exist subtypes that determine oncogenecity of the genotype.
Aims: In Slovenia, the distribution of individual high-risk genotypes in cervical cancer has not been made known yet. Also, there are no data on subtypes of these genotypes active in our area. The aim of the proposed project is to determine the distribution of HPV genotypes present in Slovenian women with cervical cancer, and to find the impact of subtypes in the development of advanced stages of the disease.
Methods: It is estimated to prove the presence of DNA HPV genotypes in 300-400 samples of cervical cancer tissue obtained at surgical treatment, and identify the subtypes of three most frequent HPV genotypes using automated sequencing.
Expected results: We expect to HPV 16 and 18 to have the highest frequency in Slovenian cervical cancer samples followed by HPV 45 and 31. In 3 most frequent genotypes, their subtypes and the impact on the disease stage and prognosis will be analyzed. Additionally, the findings will serve as a means for a more precise and more targeted decision regarding the national HPV vaccination programme in our geographic entity.
Significance for science
Distribution of human papillomavirus (HPV) genotypes in cervical cancer in Slovenia have been determined for the first time. Our results will be important for the global distribution of HPV in cervical cancer, since such data was lacking for Slovenia until now.
The analysis of genomic variants of HPV 16, HPV 18 and HPV 33 is important globally, since numerous new nucleotide substitutions have been found, especially in genomic regions LCR of those three genotypes. Genomic variants of analyzed HPV genotypes have been phylogenetically determined, as well.
Significance for the country
The distribution of human papillomavirus (HPV) genotypes in cervical cancer, which has been determined just before the introduction of HPV vaccination, will be important in the future to evaluate the effect of HPV vaccination on distribution of HPV genotypes in cervical precancerous and cancerous changes.
Most important scientific results
Annual report
2008,
2009,
final report,
complete report on dLib.si
Most important socioeconomically and culturally relevant results
Annual report
2008,
2009,
final report,
complete report on dLib.si
