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Projects / Programmes source: ARIS

Molecular biotechnology: from dynamics of biological systems to applications

Periods
Research activity

Code Science Field Subfield
4.06.00  Biotechnical sciences  Biotechnology   
1.05.00  Natural sciences and mathematics  Biochemistry and molecular biology   
1.09.00  Natural sciences and mathematics  Pharmacy   
3.01.00  Medical sciences  Microbiology and immunology   

Code Science Field
T490  Technological sciences  Biotechnology 
B230  Biomedical sciences  Microbiology, bacteriology, virology, mycology 
B510  Biomedical sciences  Infections 
B725  Biomedical sciences  Diagnostics 
P310  Natural sciences and mathematics  Proteins, enzymology 

Code Science Field
3.04  Medical and Health Sciences  Medical biotechnology 
Keywords
molecular basis of disease, lipopolysaccharide, cellular receptors, signal transduction, sepsis, biosensors, antibodies, biotechnology, prions, antimicrobial compounds, primary metabolism, structural biology, molecular modeling
Evaluation (rules)
source: COBISS
Researchers (59)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  33206  PhD Darjan Andrejc  Biology  Junior researcher  2010 - 2014  19 
2.  29065  Matevž Avbelj  Biotechnology  Technical associate  2009 - 2011 
3.  29495  PhD Monika Avbelj  Biotechnology  Researcher  2009 - 2014  55 
4.  25835  PhD Tanja Bagar  Biochemistry and molecular biology  Junior researcher  2009  256 
5.  14360  PhD Mojca Benčina  Biotechnology  Researcher  2009 - 2014  392 
6.  33205  PhD Sabina Božič Abram  Biochemistry and molecular biology  Junior researcher  2010 - 2014  42 
7.  18675  Robert Bremšak    Technical associate  2009 - 2014  11 
8.  37380  PhD Karen Butina Ogorelec  Microbiology and immunology  Technical associate  2014  34 
9.  37381  PhD Miša Mojca Cajnko  Chemical engineering  Technical associate  2014  51 
10.  25730  PhD Anja Colja Venturini  Pharmacy  Researcher  2009 - 2014  17 
11.  24259  PhD Slavko Čeru  Biochemistry and molecular biology  Researcher  2014  55 
12.  05236  PhD Vladka Čurin Šerbec  Microbiology and immunology  Researcher  2009 - 2014  261 
13.  33301  Tibor Doles  Biochemistry and molecular biology  Technical associate  2011 - 2014  41 
14.  30848  PhD Ota Fekonja  Pharmacy  Researcher  2010 - 2013  25 
15.  34529  PhD Vida Forstnerič  Biotechnology  Junior researcher  2011 - 2014  40 
16.  07731  PhD Jožefa Friedrich  Biotechnology  Researcher  2009 - 2012  184 
17.  32254  PhD Rok Gaber  Biotechnology  Technical associate  2010 - 2014  52 
18.  31950  PhD Petra Galer  Chemistry  Researcher  2014  27 
19.  28558  PhD Jernej Gašperšič  Biochemistry and molecular biology  Researcher  2009 - 2010  62 
20.  36426  PhD Anja Golob Urbanc  Biotechnology  Junior researcher  2013 - 2014  32 
21.  17915  PhD Helena Gradišar  Biotechnology  Researcher  2009 - 2014  130 
22.  23563  PhD Iva Hafner Bratkovič  Neurobiology  Researcher  2009 - 2014  210 
23.  15978  PhD Katrina Hartman  Microbiology and immunology  Researcher  2009 - 2014  43 
24.  28881  PhD Karolina Ivičak Kocjan  Biotechnology  Researcher  2009 - 2014  54 
25.  06628  PhD Roman Jerala  Biochemistry and molecular biology  Head  2009 - 2014  1,189 
26.  36853  PhD Lucija Kadunc Polajnar  Neurobiology  Junior researcher  2014  43 
27.  34520  PhD Vid Kočar  Pharmacy  Junior researcher  2011 - 2014  35 
28.  31854  PhD Tomaž Koprivnjak  Biotechnology  Researcher  2009 - 2011  26 
29.  25973  PhD Simon Koren  Biochemistry and molecular biology  Researcher  2009 - 2010  88 
30.  28420  PhD Miha Kosmač  Microbiology and immunology  Researcher  2011 - 2014  13 
31.  35344  PhD Valerija Kovač  Medical sciences  Junior researcher  2012 - 2014  20 
32.  29494  PhD Alenka Kužnik Krajnik  Pharmacy  Researcher  2009 - 2012  19 
33.  34069  PhD Duško Lainšček  Biotechnology  Junior researcher  2012 - 2014  148 
34.  34252  Tina Lebar  Biochemistry and molecular biology  Technical associate  2011 - 2014  67 
35.  09354  PhD Matic Legiša  Biotechnology  Researcher  2009 - 2014  272 
36.  33323  PhD Anja Lukan  Biochemistry and molecular biology  Junior researcher  2010 - 2014  17 
37.  17917  PhD Andreja Majerle  Biotechnology  Researcher  2009 - 2014  92 
38.  21426  PhD Mateja Manček Keber  Pharmacy  Researcher  2009 - 2014  159 
39.  23939  PhD Martina Mohorčič  Biotechnology  Researcher  2009 - 2014  30 
40.  32114  PhD Jerneja Mori  Biochemistry and molecular biology  Researcher  2009 - 2014  39 
41.  29496  Blaž Nemec  Chemistry  Junior researcher  2009 - 2011  23 
42.  29991  Alja Oblak  Biochemistry and molecular biology  Technical associate  2009 - 2014  60 
43.  17280  Darija Oven    Technical associate  2009 - 2014 
44.  26163  PhD Gabriela Panter  Biotechnology  Researcher  2009 - 2014  45 
45.  37589  PhD Tina Tinkara Peternelj  Microbiology and immunology  Researcher  2014  39 
46.  26500  PhD Nina Pirher  Biotechnology  Researcher  2009 - 2014  42 
47.  32113  PhD Jelka Pohar  Biotechnology  Beginner researcher  2009 - 2014  97 
48.  12060  PhD Primož Pristovšek  Chemistry  Researcher  2009 - 2014  135 
49.  26501  PhD Nuša Resman  Pharmacy  Researcher  2009 - 2012  13 
50.  31915  PhD Nejc Rojko  Biochemistry and molecular biology  Beginner researcher  2014  29 
51.  33201  PhD Anže Smole  Biotechnology  Junior researcher  2010 - 2014  74 
52.  29086  Boštjan Smrekar  Microbiology and immunology  Technical associate  2009 - 2014  17 
53.  29490  MSc Andreja Šmerc  Biochemistry and molecular biology  Junior researcher  2009 - 2010  10 
54.  34253  Jernej Turnšek    Technical associate  2011 - 2012  22 
55.  26515  PhD Aleksandra Usenik  Biochemistry and molecular biology  Junior researcher  2009 - 2010  54 
56.  25436  PhD Jožica Vašl  Natural sciences and mathematics  Researcher  2009 - 2014  37 
57.  21335  PhD Tanja Vranac  Biochemistry and molecular biology  Researcher  2009 - 2014  40 
58.  28563  PhD Mojca Zelnikar  Pharmacy  Junior researcher  2009  15 
59.  25437  PhD Mateja Zorko Kern  Pharmacy  Researcher  2009 - 2012  40 
Organisations (2)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0104  National Institute of Chemistry  Ljubljana  5051592000  20,958 
2.  0311  Blood Transfusion Centre of Slovenia  Ljubljana  5053960  1,737 
Abstract
Life sciences experience the transition from molecular to the systemic level, fostering the development of a new quality in our understanding of complex biological systems such as immune and metabolic system. Program connects basic research with application in the areas of research, which have primarily relevance to the health. The emphasis of the program is on the understanding of the response of the human organism to bacterial infections and conformational diseases at the molecular level and understanding of the microbial metabolism directed towards the improvement of the various pharmaceutically interesting products. Within the research of the LPS receptors we will investigate the extracellular (MD-2, TLR4, CD14) as well as intracellular receptors. Understanding of interactions at the molecular level can lead to new compounds, which might be used to neutralize the excessive immune response. The aim of this research is to elucidate the biochemical specificity of the so called “PAMP receptors”, i.e. receptors that recognize the molecular patterns of pathogenic microorganisms. Based on the structure-activity relationship (SAR) we plan to develop new (lipo)peptides and establish the differences in the selectivity between bacterial and eukaryotic membranes. In the area of health we are also investigating diseases, particularly transmissible spongiform encephalopathies (TSE) connected to conformational changes of proteins. Monoclonal antibodies have been established as diagnostic tool as well as for the therapy. By the combination of immunochemistry with methods of protein and structural biochemistry we made the first step analyzing the specificity of antibodies that recognize the pathogenic form of prion protein (PrPSc), linked to Creutzfeld-Jakob disease. This and other tools will allow us to improve the detection and early diagnostics of disease and in parallel to contribute to the understanding of the infectivity of TSE. Program also involves the research of primary metabolism of industrial microorganisms, particularly 6-phosphofructo-kinase (pfkA), whose properties significantly change during the posttranslational modification. We intend to transfer the shortened gene encoding more active enzyme into other commercially important microorganisms. We will develop the new fluorescent intracellular biosensors to monitor the events particularly in eukaryotic cells. We will put the emphasis on the analysis of Ca, cAMP and pH inside of living cells.
Significance for science
Achievements of the program Molecular Biotechnology represent an important contribution of science in understanding the molecular mechanism of innate immunity signaling, which advanced significantly in the last decade. In addition to understanding the basic mechanisms of recognition and signaling the results are relevant for a wide range of pathological phenomena from infections, chronic inflammation to cancer and for understanding and improving the treatment of these diseases. Members of the program group significantly shaped the development of synthetic biology in the world in recent times. The group introduced a concept of designable modularity, which means the use of protein domains that can be prepared in a number of variants, with the help of which one can make a new modular structures or introduce the cell logic. Such modular building components are coiled-coil dimers for the preparation of new types of protein folding process which defines the topology, which is probably the most original contribution of this group to science in the last period. Preparation of protein tetrahedron experienced a remarkable recognition in the scientific community. The second group of modular building blocks are DNA binding proteins based on TALE domains, which enabled preparation of orthogonal NOR gates and bistable gene switch. Additionally, we can highlight the design of biosynthesis frame on the substance of DNA and DNA binding domain which improved the production of three reactions and has been followed by other scientists.
Significance for the country
Research program Molecular Biotechnology is important for Slovenia because it is among the leading groups in research of innate immunity and synthetic biology. The program allows contact with the cutting edge science on these two areas, which are important for health as well as the increasingly important for the technology. The program group has the important role in training qualified researchers who are able to solve complex scientific and developmental problems. The success of the group in the field of science and higher education and intensive international involvement are important for the promotion of Slovenia as a developed country and an important partner. Cooperation with companies in the field of high technology allows the transfer of scientific advances in industrial applications with partners in Slovenia and around the world. The group is also important for the promotion of science as a key factor in the successful development and part of the intellectual identity in Slovenia at the level of secondary school students, their mentors, students, researchers, politicians and the general public.
Audiovisual sources (1)
no. Title (with video link) Event Source
1. Molecular biotechnology: from dynamics of biological systems to applications    Research programme video presentation 
Most important scientific results Annual report 2009, 2010, 2011, 2012, 2013, final report, complete report on dLib.si
Most important socioeconomically and culturally relevant results Annual report 2009, 2010, 2011, 2012, 2013, final report, complete report on dLib.si
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