Projects / Programmes
Uravnavanje koproteazne aktivnosti proteina RecA v bakterijah (Slovene)
| Code |
Science |
Field |
Subfield |
| 1.05.00 |
Natural sciences and mathematics |
Biochemistry and molecular biology |
|
Researchers (1)
| no. |
Code |
Name and surname |
Research area |
Role |
Period |
No. of publicationsNo. of publications |
| 1. |
24290 |
PhD Matej Butala |
Biochemistry and molecular biology |
Head |
2009 - 2011 |
236 |
Organisations (1)
Significance for science
The bacterial SOS response is essential for the maintenance of genomes, but also modulates antibiotic resistance. Our results provide insights into the mechanisms underlying SOS response and are prerequisite to understand the mechanism behind programmed expression of the LexA regulon genes. Hence, this work sets a novel platform for drug discovery to treat bacterial pathogens and offers an approach to control bacterial survival of antibiotic therapy.
I have established international colaborations in order to carry out this project. I have collaborated with prof. Steinhoff`s group from Germany. We have applied EPR methods to LexA. We continued collaboration with the group of prof. Steve Busby and established collaboration with dr. David Grainger from the UK. Collaborations established in Slovenia: Infrastractural centre for surface plasmon resonance, measurements performed in collaboration with prof. Gregor Anderluh and Vesna Hodnik; for development of anti-LexA compounds I collaborated with dr. Mojca Lunder and the group of prof. Stanislav Gobec, Faculty of Pharmacy, UL. We continued collaboration with dr. Milan Hodošček, Chemical institute, Ljubljana. International colaboration in this project can be observed from the joint publications.
This research project focused on deepening and broadening the understanding of bacterial gene regulation due to stress response in bacteria and their influence on phenomena of antibiotic resistance. One of the main added values to the European research community lies in increasing the potential of Slovenia as a centre for fundamental research in molecular microbiology. The area of antimicrobial stress response is highly competitive internationally. There are rapidly developing centres of excellence in this research area within Japan, China and US. This project established the international community in this field and promoted its general ability to make high impact research contributions to further European Excellence. Thus, after this project is finished we will continue collaborating on the SOS response with the above mentioned research groups.
Significance for the country
Results obtained from this project elucidate how bacteria respond to the environmental stress, promote bacterial evolution, which is important for further studies on the SOS response and presents a model for textbooks for the students. The results from this project will benifit to the recognition of Slovenian science abroad as the results are /will be published in a high impact journals and in the encyclopedia.
Part of this project was performed by Ana Rems uni. dipl. microbiol. (currently a PhD student at Technical University of Denmark), Tanje Đapa uni. dipl. microbiol. (currently a PhD student in Novartis, Siena), results from this project were used for their graduation thesis. Thus, this project established two talented Slovanian young scientist.
As the treatments to treat bacterial pathogens are narrowing, new methods are needed.
The set up collaborations and the obtained results enabled us to elucidate the important insights into the molecular mechanism of the bacterial response to antibiotics. Thus, development of an efficient inhibitor that will block SOS response and prevent development and spread of antibiotic resistance genes among bacteria, will hopefuly allow pharmaceutical companies to maintain production of clinically significant antibiotics, which is of great importance for the Slovenian economy.
Most important scientific results
Annual report
2009,
2010,
final report,
complete report on dLib.si
Most important socioeconomically and culturally relevant results
Annual report
2009,
2010,
final report,
complete report on dLib.si
