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Projects / Programmes source: ARIS

Development and in vitro characterization of multimodal magnetic nanoparticles for drug delivery and cell tracking

Research activity

Code Science Field Subfield
2.04.00  Engineering sciences and technologies  Materials science and technology   

Code Science Field
T150  Technological sciences  Material technology 

Code Science Field
2.10  Engineering and Technology  Nano-technology 
Keywords
magnetic nanoparticles, drug delivery, cell labeling, nanotoxicology, in vitro
Evaluation (rules)
source: COBISS
Researchers (16)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  36454  PhD Maruša Bizjak  Biochemistry and molecular biology  Junior researcher  2014 - 2017  30 
2.  19411  PhD Vladimir Boštjan Bregar  Materials science and technology  Head  2014 - 2017  105 
3.  37636  Klemen Dolinar  Medical sciences  Technical associate  2015 - 2017  65 
4.  15873  PhD Mateja Erdani Kreft  Neurobiology  Researcher  2014 - 2017  407 
5.  23563  PhD Iva Hafner Bratkovič  Neurobiology  Researcher  2014 - 2017  207 
6.  06628  PhD Roman Jerala  Biochemistry and molecular biology  Researcher  2014 - 2017  1,182 
7.  34385  PhD Jasna Lojk  Metabolic and hormonal disorders  Researcher  2014 - 2017  68 
8.  27526  PhD Maruša Lokar  Biochemistry and molecular biology  Researcher  2014  37 
9.  39583  Petra Malavašič  Biochemistry and molecular biology  Technical associate  2016 - 2017  32 
10.  24724  PhD Branka Mušič  Civil engineering  Researcher  2014 - 2017  109 
11.  17280  Darija Oven    Technical associate  2014 
12.  19225  PhD Mojca Pavlin  Systems and cybernetics  Researcher  2014 - 2017  262 
13.  33137  PhD Matej Skočaj  Biochemistry and molecular biology  Researcher  2015 - 2016  106 
14.  37508  PhD Klemen Strojan  Biotechnology  Junior researcher  2016 - 2017  25 
15.  08279  PhD Peter Veranič  Neurobiology  Researcher  2014 - 2017  358 
16.  32009  PhD Tanja Višnjar  Human reproduction  Researcher  2014  45 
Organisations (3)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0104  National Institute of Chemistry  Ljubljana  5051592000  21,475 
2.  0381  University of Ljubljana, Faculty of Medicine  Ljubljana  1627066  46,268 
3.  1538  University of Ljubljana, Faculty of Electrical Engineering  Ljubljana  1626965  27,588 
Abstract
In recent decades nanoparticles (NP) based delivery systems emerged. Magnetic nanoparticles (MNPs) due to their multimodal properties can enable targeted drug delivery combined by visualization. Still development of adequate nanoparticles as carrier system  for drug delivery presents a chalenge:  NPs have to be biocompatible, stable in physiological conditions, have to enable sufficient loading capacity and specific release in target cells and should preferentially enable. Finally, the toxicity has to be either minimal (for cell labelling) or specific in case of delivery systems for e.g. cancer therapy. Multimodal MNPs with possibility of high intracellular loading would present an excellent system for different applications, thus we will build on so far acquired knowledge of synthesis, functionalization, characterization in order to further develop appropriate MNPs for labeling and drug delivery applications. Further development of specifically designed MNPs together with protocols for quantification and visualization of NPs interactions with cells and subcellular compartments will open new possibilities in field of nanotoxicology and drug delivery, while better understanding of endocytotic pathways, intracellular fate and permeation of NPs through barriers will assist in development and optimisation of new MNPs for specific applications. Further, long-term nanocytotoxicity studies are crucial for NPs based applications. The project will bring both new protocols for assessing long-term cytotoxicity on realistic biomimetic in vitro models, as well as gain us new insights in analysis of how environmental and biomedically relevant NPs interact and affect the cells. Immediate and long-term toxicity, changes in cell proliferation, generation of reactive oxigen species and immune response will be analyzed in order to obtain clear pictures of relevant mechanisms. Strong collaboration within interdisciplinary partner groups will enable us to gain new scientific knowledge regarding NPs interactions, toxicity and related mechanisms, as well as to transfer the obtained knowledge directly to applications and/or clinical environment.
Significance for science
We have focused on development of magnetic nanoparticles (MNPs) suitable for visualization an drug delivery. Developed multimodal MNPs together with protocols for quantification and visualization of NPs interactions with cells and subcellular compartments open new possibilities in field of cell labeling and drug delivery, while better understanding of endocytotic pathways, intracellular fate and permeation of NPs through barriers will assist in development and application of new MNPs for drug delivery. Further, long-term nanocytotoxicity studies are crucial for NPs based applications. Within the project we developed new protocols for assessing long-term cytotoxicity. In additoin we gained new insights in analysis of how environmental and biomedically relevant NPs interact and affect the chosen cell lines. Cell cycle, proliferation, differentiation, immune response immediate toxicity, ROS generation were analyzed in order to obtain clear pictures of mechanisms behind. Strong collaboration with many partners enabled us to gain new scientific knowledge regarding NPs interactions, toxicity and related mechanisms. Transdisciplinary approach integrated in one project team enabled us integration of new knowledge from diverse scientific fields of physics, material sciences and cell biology. Importantly, the observed highly selective uptake of nanoparticles into cancer urothelial cells opens a posibility for selective targeting
Significance for the country
Possible end users of the project results are both biotechnology and pharmaceutical companies as well as medical and research organizations. The results of the project will aid to new protocols for synthesis and functionalisation of advanced nanoparticle, to better understanding of a nanoparticle behavior in the biological systems, and finally to new protocols and better understanding of the long-term effects of NPs on biological systems. One of the major goals was to develop methods for synthesis, functionalization and characterization of nanoparticles as drug delivery systems or for visualization. Magnetic NPs for cell labeling and tracking will aid to faster translation of different methods of tissue regeneration based on transplantation of specific types of cells (e.g. stem cells).
Most important scientific results Annual report 2014, 2015, final report
Most important socioeconomically and culturally relevant results Annual report 2014, 2015, final report
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