Tissue proteinases and their inhibitors: Characterisation, genetic engineering and structure-function relationship

Code

J1-7422 (D) - included in ARIS records

Head

Period

1/1/1996 - 6/30/2001

Science

Natural sciences and mathematics (15)
Other (1)

Reseacher status

Researcher (12)
Junior expert or technical associate (4)

Education

Doctor's degree (12)
Other (4)

Sex

Woman (10)
Man (6)

Status

Employed at RO (1)
Employed at RO and RRD (10)
No data on employment in RO (2)
Retired (2)
Deceased (1)

No. of publications

10–99 (7)
100–999 (7)
1,000–9,999 (2)

Projects / Programmes source: ARIS

source: ARIS

Tissue proteinases and their inhibitors: Characterisation, genetic engineering and structure-function relationship

Research activity

Code	Science	Field	Subfield
1.05.00	Natural sciences and mathematics	Biochemistry and molecular biology

Code	Science	Field
P004	Natural sciences and mathematics	Biochemistry, Metabolism

Keywords

cathepsins, cysteine proteases, cystatins, thyroglobulin type-1 domain, antigen presentation, equistatin, thyropin, invariant chain of MHC class-II molecules, inhibitors, cathepsin L, cathepsin S, cathepsin H, cathepsin B, cancer

Evaluation (rules)

source: COBISS

Evaluation of bibliographic research performance indicators according to ARIS methodology

Citations Citations for bibliographic records in COBIB.SI that are linked to records in citation databases

source: WoS

source: Scopus

source: COBISS

Researchers (16)

no.	Code	Name and surname	Research area	Role	Period	No. of publicationsNo. of publications
1.	12047	PhD Tadeja Bevec	Pharmacy	Researcher	2000 - 2001	36
2.	15970	Andreja Doberšek	Biochemistry and molecular biology	Researcher	1996 - 2001	25
3.	00449	PhD Iztok Dolenc	Biochemistry and molecular biology	Researcher	2000 - 2001	110
4.	06416	PhD Marko Dolinar	Biochemistry and molecular biology	Researcher	2000 - 2001	341
5.	10502	PhD Nataša Kopitar Jerala	Biochemistry and molecular biology	Researcher	1996 - 2001	239
6.	17110	Louisa Johanna Kroon Žitko		Researcher	1996 - 2001	22
7.	13598	PhD Robert Kuhelj	Biochemistry and molecular biology	Researcher	1996 - 2001	37
8.	03422	PhD Brigita Lenarčič	Biochemistry and molecular biology	Researcher	1999 - 2001	338
9.	06056	PhD Metka Renko	Biochemistry and molecular biology	Researcher	2000 - 2001	99
10.	14829	PhD Veronika Stoka	Biochemistry and molecular biology	Researcher	1996 - 2001	237
11.	15969	Ivica Štefe	Biochemistry and molecular biology	Researcher	1996 - 2001	36
12.	05234	Mojca Trstenjak Prebanda	Biochemistry and molecular biology	Researcher	2000 - 2001	64
13.	07561	PhD Boris Turk	Biochemistry and molecular biology	Researcher	1996 - 2001	1,037
14.	04988	PhD Dušan Turk	Biochemistry and molecular biology	Researcher	2000 - 2001	622
15.	01085	PhD Vito Turk	Biochemistry and molecular biology	Head	1998 - 2001	1,490
16.	03368	PhD Eva Žerovnik	Biochemistry and molecular biology	Researcher	2000 - 2001	389

Organisations (1)

no.	Code	Research organisation	City	Registration number	No. of publicationsNo. of publications
1.	0106	Jožef Stefan Institute	Ljubljana	5051606000	90,742

Abstract

The proposed project deals with biochemical studies of cysteine proteinases, mostly of human origin, with emphasys on cathepsins B, H, L, S and C and cruzipain from T. cruzi. Studies were performed on natural and recombinant proteins. Refolding of recombinant sulphonated procathepsin S from 6M urea by kinetic stopped-flow measurements were studies. pH-induced conformational transitions of the inhibitory propeptide of human cathepsin L contribute to further understanding of the mechanism of zymogen activation at acidic pH. Porcine spleen cathepsin H was isolated, its crystal structure was determined and its aminopeptidase activity explained. Based on known kinetics and structural data the revised definition of substrate binding sites of papain-like cysteine proteinases was proposed. The oligomeric nature of human cathepsin C was investigated. Studies on the role of cathepsins and their inhibitors stefins in prognosis, diagnosis and cancer therapy were continued. The folding of stefin A and B under denaturating conditions were studied. The discovery of invariant chain (Ii) of MHC class-II molecules and equistatin was essential for discovery of thyropins, a new superfamily of protein inhibitors of cysteine proteinases. Colorado potato beetle larve on equistatin-coted potato leaves were strongly retarded in their growth indicating high potential in biotechnology.