Projects / Programmes source: ARIS

Human Reproduction Experimental And Laboratory Aspects

Research activity

Code Science Field Subfield
3.05.00  Medical sciences  Human reproduction   
1.03.00  Natural sciences and mathematics  Biology   

Code Science Field
B570  Biomedical sciences  Obstetrics, gynaecology, andrology, reproduction, sexuality 

Code Science Field
3.02  Medical and Health Sciences  Clinical medicine 
fertilizationfailure, sperm chromatin, tubulin, androgenes, FSHR, PCR, gene expression, time-lapse, embryo quality, ovarian poor-responder, MAR, laparoscopy, AMH, malignant gliomas, biopsy, microbiological analysis, PCR
Evaluation (rules)
source: COBISS
Researchers (14)
no. Code Name and surname Research area Role Period No. of publicationsNo. of publications
1.  14113  PhD Igor But  Human reproduction  Researcher  2015 - 2019  468 
2.  35345  PhD Maruška Čuš  Human reproduction  Junior researcher  2015 - 2017  19 
3.  25129  PhD Aleš Goropevšek  Microbiology and immunology  Researcher  2019  55 
4.  29934  Nina Hojnik    Technical associate  2015 - 2019  30 
5.  33092  PhD Jure Knez  Human reproduction  Researcher  2015 - 2019  187 
6.  15712  Martina Kos  Public health (occupational safety)  Technical associate  2015 - 2019  39 
7.  20136  PhD Vilma Kovač  Human reproduction  Researcher  2015 - 2019  158 
8.  11040  PhD Borut Kovačič  Human reproduction  Head  2015 - 2019  368 
9.  28993  Jasna Muršič    Technical associate  2015 - 2019 
10.  39084  PhD Erika Prašnikar  Human reproduction  Junior researcher  2016 - 2019  13 
11.  15711  PhD Milan Reljič  Human reproduction  Researcher  2015 - 2019  292 
12.  17002  PhD Tadej Strojnik  Oncology  Researcher  2015 - 2017  230 
13.  37828  Marjan Taborin    Technical associate  2015 - 2019  16 
14.  21099  PhD Branka Žegura  Human reproduction  Researcher  2018 - 2019  324 
Organisations (1)
no. Code Research organisation City Registration number No. of publicationsNo. of publications
1.  0334  University Medical Centre Maribor  Maribor  5054150000  23,020 
1. There is no data about defects of function of the sperm astralmicrotubules during fertilization process. In donated human oocytes which failed to fertilize after intracytoplasmic sperm injection (ICSI), tubulin and chromatin will be stain, and the synchronization of microtubulerising and sperm head decondenzation will be observed. Disorders in synchronization of these processes will be analysed in cases of fertilization failure.   2. The beneficial effect of artificial shrinkage of blastocyst before cryopreservation for survival and implantation has not been confirmed by any randomized clinical trial yet. Blastocysts will be vitrified by using the conventional vitrification protocol. After blastocyst warming, the survival and implantation rates will be compared between study and control groups. 3. Influence of androgen in mediating FSH action on the expression of FSHR on granulosa cells will be studied in patients with poor respond on ovarian hyperstimulation which where treated with medically asssisted reproduction.We want to confirm the positive effect of androgens on FSHR gene expression which will be confirmed whith randomised controled trial. Similar studies are not published yet. 4. Time-lapse monitoring of early development of embryos derived from poor-responder patients with impaired response to controlled ovarian hyperstimulation. Analysis of events in early embryo development  in vitro and detection of major abnormalities (aberrant cell divisions, reverse in number of cells, fragmentation). 5. In this study we are going to determine the blood level of AMH in all consecutive patients in reproductive years who will undergo ovarian surgery due to endometriomas or other cysts (dermoid and serous cysts) or pathological conditions (PCO). The AMH level will be detrermined before and after surgery with aim to assess the sensitivity of the test for determination of the decreased postoperative ovarian reserve. At the same time we wil try to estimate whether it is feasible to assess the quality and proffesionalism of performed surgery. 6. Gliomas are the most common form of brain tumors, contributing about half of the incidence of brain tumors. The impact of microorganisms is one of the possible risk or etiological factors. We are going to study the presence of microorganisms in the samples of the malignant gliomas using the microbiological analysis using PCR method. Results will contribute to knowledge of infection as important factor in development of malignant gliomas.
Significance for science
The studies will provide new information about celullar mechanisms of fertilization and gamete deffects causing fertilization failure.   By randomized clinical trial, it will be showed, if the artificial shrinkage before vitrification of blastocysts is reasonable. The hypothesis of  the androgen influence on granulosa cell FSH receptor system will be tested by genetic analysis of FSH receptors in poor ovarian respnders to FSH stimulation. The confirmation of hypothesis by our study, a completely new approach in the treatment of infertility in poor responders could be introduced.   The underlying mechanisms of how age or poor ovarian reserve affects the quality of embryos are not very well established. Time-lapse technology for evaluation of in vitro embryo development enables us detection and possible explanation of cleavage abnormalities that are otherwise invisible by single daily observation of embryos with light microscope. The study of AMH in the ovary after surgery is important for assessing the fertility potential of women after surgery. The results of prognostic markers research help us evaluate brain lesions. This improves treatment plans for individual patients. New facts about the potential role of the infection in the glioma pathogenesis would significantly influence glioma patients’ treatment. The effective treatment of the infection may interfere with carcinogenesis or even prevent cancer.
Significance for the country
Patients with impaired response to controlled ovarian hyperstimulation represent very problematic group of women with fertility problems. According to some data, one third of infertile women are poor-responders. Knowing the reasons for low success rate in this group, might help to find new alternatives, more appropriate from psychological or socioeconomical aspect. It is also very important that science based information of effect of reproductive age is available for women that tend to have children at higher age. Results of the research will be particularly useful for those patients, which represent significant financial burden for the health fund because of poor response to FSH and consequently low success of ART. Cancer patients represent social and economic burden in our society, therefore every improvement in the management and treatment of them has positive effects.
Most important scientific results Annual report 2015, interim report, final report
Most important socioeconomically and culturally relevant results Annual report 2015, interim report, final report
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