Professor I. Mandic Mulec was invited to give a key note at the international conference "Interbacterial cooperation nd competition" organized by American society of microbiology in Washington, where she presented common published results on kin discrimination , discovery which resulted from productive collaboration between Mandič-Mulec team and Kolter lab at Harvard. The results are highly relevant for the project as they support one pof the hypothesis that interactions between non-kin involve antagonisms. Project elader has been invted to lecture at famous universities abroad [COBISS.SI-ID 4875896], [COBISS.SI-ID 4759160], [COBISS.SI-ID 4692856], [COBISS.SI-ID 4752504] , [COBISS.SI-ID 4649848] and at interantional workshops [COBISS.SI-ID 4992632] [COBISS.SI-ID 4817784] [COBISS.SI-ID 4752760], [COBISS.SI-ID 4734840] [COBISS.SI-ID 4692344]. She is an invited speaker at the most important microbiology conference in Europe- FEMS conference, July 2019 ; where she organizes also a section on social ecology and will present achievements of this project.
B.04 Guest lecture
COBISS.SI-ID: 4762488The project leader prof. Ines Mandić Mulec was recognized at the national level for her excellence in science - specifically for her achievements in the field of microbiology. After the gala even she was invited for an interview in the prime time news at the national television where she stated her views on developments in Science in Slovenia, presented briefly her research filed of social microbiology and microbial ecology and stressed the urgent need to increase state funds for Slovenian research.
E.01 National awards
COBISS.SI-ID: 4878712Project leader Ines Mandič Muec is a member of the editorial board of the scientific journal Biofilm published by Elsevier. https://www.journals.elsevier.com/biofilm/news/meet-the-editors-of-biofilm
C.06 Editorial board membership
COBISS.SI-ID: 5030264In connection with the project, David Vogrinc has done his master thesis in the laboratories of Acies Bio and in cooperation with the Biotechnical Faculty. Within the master's thesis he evaluated several approaches for inactivating gene clusters for the biosynthesis of secondary metabolites in Streptomyces rapamycinicus. Among other things, he tested the Crispr / Cas9 approach. Through his work, he further contributed to the expertise of Acies Bio in the genetic manipulation of this complex organism, and improved the possibilities for identifying novel compounds, biosynthesized by silent gene clusters. In addition to this master theses in the scope of the project were mentored by members of the team and defendedin 2018; Nataša Jersinovič, [COBISS.SI-ID 4921208], mentor IMM, somentor Dogša Iztok; 2018 Monika Botulen [COBISS.SI-ID 4959864],mentor IMM, somentor Barbara Kraigher; 2016 Štampar Martina [COBISS.SI-ID 8481145] mentor IMM, somentor B. Kraigher; 2018 Cerovečki Ana [COBISS.SI-ID 4844920]mentor B. Kraigherr; 2018 Černoša Anja[COBISS.SI-ID 4989816], mentor T. Danevčič, somentor P. Jamnik; 2019 LOGAR, Erika [COBISS.SI-ID 5019000] mentor Anja Klančnik; 2018 KRAPEŽ, Katarina [COBISS.SI-ID 4961656], mentor Anja Klančnik
D.10 Educational activities
COBISS.SI-ID: 9053305Campylobacter jejuni is one of the most prevalent causes of bacterial gastroenteritis worldwide. With the aim to combat its increasing antimicrobial resistance, we introduce alternative strategy to control the C. jejuni growth using the co-culturing with Bacillus subtilis. We successfully reduce fifteen C. jejuni strains of human, slaughter, and water origin in the range of reduction from 2.8 to 4.2 log CFU/mL, regardless the ration of co-culture inoculum used. To further test whether growth inhibition was due to B. subtilis competition or antagonism, we exposed C. jejuni to B. subtilis spent medium, prepared as medium in which B. subtilis grow but with removed cells (SM). The low C. jejuni inhibition rate in SM is implying that the observed inhibitory effects of B. subtilis are not only due to the production of antibiotics. We conclude that in environments that favor C. jejuni growth B. subtilis is a strong competitor of this pathogen, efficiently controlling its numbers even when the initial frequency of C. jejuni exceeds B. subtilis greatly. This inhibitory effect is not observed in environments where C. jejuni growth is limited by environmental factors (e.g. oxygen).
B.03 Paper at an international scientific conference
COBISS.SI-ID: 4961400