Since campylobacteriosis is the leading bacterial food-borne illness and antimicrobial resistance is prevalent, we have analyzed the prevalence of quinolone resistance among C. jejuni strains isolated from various sources (animal, food, human and environmental samples) to elucidate the nature of the quinolone resistance expansion. We performed comparative genetic similarity analyses using allelic types of seven multilocus sequence typing housekeeping genes, and single nucleotide polymorphisms of a quinolone resistance determining region located within the DNA gyrase subunit A gene of 180 resistant strains of C. jejuni from different sources. This analysis revealed high genetic similarity of isolates from clonal complex ST-21 that carry gyrA allelic type 1. To tackle the problem of bacterial resistance, natural antimicrobials, efflux inhibitors and plant resistance modulators have been studied and mechanisms of their resistance-modifying action in C. jejuni were overviewed in the invited lecture on international microbiological conference.
B.04 Guest lecture
COBISS.SI-ID: 4657784Modern diagnostic and research laboratories have the equipment and expertise to perform analyses by traditional methods in combination with molecular and serological methods. Molecular methods are most rapidly developing among the modern diagnostic methods. In comparison with conventional methods, molecular methods are faster and, most importantly, enable also the analysis of microbes that cannot grow under laboratory conditions or grow very slowly. Among molecular methods, PCR with its variants and sequencing are the most commonly used. After sequencing of the first-generation (Sanger), the next-generation sequencing (NGS) has been gaining popularity in the last decade as offering the most comprehensive diagnostic and research answers. With NGS, a large number of data is collected, but only after proper analyses with specialized bioinformatic tools they show a practical value. Among the newer methods, the MALDI-TOF mass spectrometry is also coming into the routine use. It is a method that is affordable for rapid and routine identification of bacterial isolates in clinical microbiological laboratories. In addition, methods that enable simultaneous analysis of a large number of expressed characteristics are also being promoted, e.g. the phenotypic microarrays and the automated multiparametric immunoassay systems.
B.04 Guest lecture
COBISS.SI-ID: 4240250Campylobacteriosis as food-born zoonoses is most commonly associated with the consumption of contaminated poultry meat. The causes of disease are C. jejuni and C. coli, followed by C. consisus, C. curves, C. upsailensis and C. lari. As the possible source of disease poultry is in the first place, considering that the prevalence of pathogens in broiler flocks is very high. Critical points for contamination of carcasses during slaughter of infected animals occur at the beginning of the slaughter line and they are present during all operation steps (cutting, retail). Previous studies and projects carried out, show that currently play the largest role in reducing the level of contamination of chicken meat improvement of bio-security measures during the breeding animals. A decisive role in reducing the number of campylobacteriosis should in any case have to play the awareness of
B.06 Other
COBISS.SI-ID: 4232826