In connection with the increase antimicrobial resistance and its consequences, the national strategy and action plan, which will stop the rise of resistance, are necessary. In 2012, the European Council invited Member States of the European Union to draw up a national strategy for the prevention of antimicrobial resistance in human and veterinary medicine under the initiative of the European Council, "One Health". The Ministry of Health has appointed an expert committee 24.11.2014 (Decision no. 2711-14-994643). Among the members of the Commission for the prudent use of antimicrobial drugs in the Ministry of Health, it was selected 5 members who have prepared a national strategy for the period 2016-2022.
B.06 OtherCOBISS.SI-ID: 3173036
In the past decade there has been a rapid increase in the number of infections with Enterobacteriaceae which produces extended-spectrum beta-lactamases (ESBL) in humans. Clones that are both resistant and virulent have been expanded. E. coli with ESBL is frequently present in farm animals, especially in poultry and chicken meat, which is also found in Slovenia. The connection between human and animal isolates is complex and monitoring of the mode of spread resistant bacteria is possible only by molecular methods. The proportions of E. coli with ESBL in humans that derived from animals are probably diffrent from country to country.
B.06 OtherCOBISS.SI-ID: 3764453
Modern diagnostic and research laboratories have the equipment and expertise to perform analyses by traditional methods in combination with molecular and serological methods. Molecular methods are most rapidly developing among the modern diagnostic methods. In comparison with conventional methods, molecular methods are faster and, most importantly, enable also the analysis of microbes that cannot grow under laboratory conditions or grow very slowly. Among molecular methods, PCR with its variants and sequencing are the most commonly used. After sequencing of the first-generation (Sanger), the next-generation sequencing (NGS) has been gaining popularity in the last decade as offering the most comprehensive diagnostic and research answers. With NGS, a large number of data is collected, but only after proper analyses with specialized bioinformatic tools they show a practical value. Among the newer methods, the MALDI-TOF mass spectrometry is also coming into the routine use. It is a method that is affordable for rapid and routine identification of bacterial isolates in clinical microbiological laboratories. In addition, methods that enable simultaneous analysis of a large number of expressed characteristics are also being promoted, e.g. the phenotypic microarrays and the automated multiparametric immunoassay systems.
B.04 Guest lectureCOBISS.SI-ID: 4240250