Predisposing factors for different types of urinary tract infections (UTI) were evaluated and prevalence of causative agents and their resistance were identified. A prospective epidemiologic study included 191 dogs with signs of urinary tract disease. Most common causative agents of UTI were E. coli, staphylococci, Proteus sp., and enterococci. The causative agents were in 29.4% susceptible to all tested antimicrobials. Resistant bacteria were more common in dogs previously treated with antimicrobials. Data on uropathogens and their resistance in regard to concurrent diseases is of crucial importance for diagnosis, treatment and prevention of complications in dogs with UT.
COBISS.SI-ID: 4264058
A significant risk factor for developing Clostridium difficile infection (CDI) in humans and animals is associated with the antimicrobial use. A total of 159 CD isolates from 20 family dairy farms were tested with a customized broth microdilution plate for their antimicrobial resistance. Seventeen antimicrobials which are commonly used for treatment of CDI in veterinary and human medicine, were selected. Most CD isolates were resistant to erythromycin, daptomycin and clindamycin. High multiple-resistance was found in CD ribotype 012, some CD SLO 060 and one CD 033. High multiple-resistance in this study was linked with CD ribotypes and not with the origin of CD. The low prevalence of these ribotypes indicates that family-run dairy farms are an unlikely source of CD with multiple-resistance to antimicrobials.
COBISS.SI-ID: 4178810
Bacterial communication (quorum sensing, QS) in Gram-negative bacteria, like Pseudomonas aeruginosa, takes place via the production and collective response to signal molecules N-acylhomoserine lactones (AHLs). P. aeruginosa is a common cause of inflammation of the ear canal (otitis externa, OE) in dogs and employs QS to coordinate the expression of virulence factors. It is often resistant to several antibiotics. Attenuation of QS signals to inhibit virulence is a novel strategy for the treatment of resistant bacterial pathogens. The purpose of this study was to validate an analytical procedure with LC-MS/MS chromatography for determination of AHLs in the ear rinses from dogs with P. aeruginosa-associated OE. With the validated procedure, AHLs were analyzed in the samples taken from two dogs, receiving a standard treatment for P. aeruginosa-associated OE. Validation was successful and a time dependent reduction of AHLs was detected for the treated dogs. This was the first report on determination of AHLs in the clinical samples of veterinary importance. The analytical procedure described is capable of supporting novel antimicrobial strategies, which target QS.
COBISS.SI-ID: 4206458