In 2016 lumpy skin disease (LSD) was rapidly spreading into several countries, causing serious challenge regarding implementation of successful control of disease and emergence of vaccination. The first case of LSD in Serbia was laboratory confirmed in Municipality of Bujanovac in June 2016. Virus was isolated in Madin-Darby bovine kidney (MDBK) cells and complete genome of LSD virus was determined from the isolate SRB/Bujanovac/2016 by next generation sequencing, using Ion Torrent technology.The determined virus contains 156 open reading frames (ORFs). The individual nucleotide changes were randomly distributed through whole viral genome. This is the first complete LSD virus genome determined in Serbia and also in Balkan area.
B.04 Guest lectureCOBISS.SI-ID: 4304506
The next-generation sequencing technology (NGS), also known as mass-parallel sequencing or sequencing of high-capacity, is becoming increasingly indispensable in virological laboratories around the world and is one of the newest approaches to identify pathogens, their characterization and the epidemiological control of infectious disease agents. An important advantage of NGS is that it is not limited by the previously known nucleotide sequence of pathogens, thus allowing direct detection of unknown pathogens and simultaneous detection of multiple pathogens in clinical samples. High capacity enables the acquisition of complete sequences of genomes of a large number of strains in a time and price acceptable range.
F.06 Development of a new productCOBISS.SI-ID: 4387194
In this invited lecture at the seminar »Next generation Sequencing in Microbiology«, organized by Kemomed, results of our project using NGS approaches for comparative genomics of C. difficile and other medically important bacteria were presented.
E.03 OtherCOBISS.SI-ID: 92616449