Individual swab samples were tested in the laboratory using three commercial and one in-house real-time PCR methods, to detect nucleic acids of a total of ten different respiratory pathogens. Pasteurella multocida (P. multocida) was detected in 58.65% of samples, Mannheimia haemolytica (M. haemolytica) in 15.04%, while Mycoplasma bovis (M. bovis) and Histophilus somni (H. somni) were positive in 9.77% of nasal swab samples. Among viral pathogens, the highest prevalence (40.60%) was observed for bovine respiratory syncytial virus (BRSV), followed by bovine coronavirus (BCV) 12.03%, bovine para-influenza 3 (PI-3) 3.01%, and bovine viral diarrhea virus (BVDV) with 1.50% of positive samples. The less frequently detected viral pathogens were bovine herpes virus type 1 (BHV-1) and bovine adenovirus (BAdV) with 0.75% positive samples each. Individual samples from this study will be used for respirartory pathogens detection by using NGS technology.
COBISS.SI-ID: 4396666
The lumpy skin disease virus (LSDV) isolate SRB/Bujanovac/2016 consists of 150.661 nucleotides and has a 99.95% nucleotide identity with Neethling Warmbaths LW strain isolated in South Africa in 1999. This is the first complete LSDV genome determined in Serbia and also in the Balkan area.
COBISS.SI-ID: 4374138
In this plenary lecture at the Congress of Slovenian Microbiological Society the part of results on comparative genomics of C. difficile was presented as well as part of the results on microbiota analysis in association with C. difficile colonization resistance.
COBISS.SI-ID: 512767288